Scanning spectrophotometer for high throughput fluorescence detection
A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.
1. A reflection light transfer module including:
(a) an input mirror, positioned substantially coaxial with an area to be illuminated, for directing incoming light to illuminate the area; and
(b) an output mirror, positioned substantially coaxial with the area to be illuminated and in reflective alignment with the input mirror, for collecting, focusing, and directing emitted light away from the area to be illuminated, wherein the emitted light is emitted by the area upon illumination.
2. The reflection light transfer module of claim 1 , wherein the output mirror is a spherical mirror.
3. The reflection light transfer module of claim 1 , wherein the input and output mirrors are first-surface mirrors.
4. The reflection light transfer module of claim 1 , further comprising an input director for directing light received at an input port to the input mirror as the incoming light.
5. The reflection light transfer module of claim 4 , wherein the input director selects a location of the input port.
6. The reflection light transfer module of claim 1 , further comprising an output director for directing the emitted light collected by the output mirror to an output port.
7. The reflection light transfer module of claim 6 , wherein the output director selects a location of the output port.
8. The reflection light transfer module of claim 4 , wherein the sample is maintained on a culture plate, the sample being positioned to receive the excitation light by translating the culture plate in a plane substantially perpendicular to the directed excitation light.
9. The reflection light transfer module of claim 1 , and further comprising an input director for receiving the incoming light from a selected input port and for directing the incoming light to the input mirror.
10. The reflection light transfer module of claim 9 , wherein placement of the input director selects the input port.
11. A reflection light transfer module comprising:
an input mirror for directing an excitation light to a sample;
an output mirror, positioned on a common axis with the input mirror and collecting an emitted light from the sample, the emitted light being emitted by the sample in response to illumination of the sample, wherein the sample is positioned substantially coaxially with the output mirror and the output mirror directs the emitted light away from the sample, and wherein the sample is maintained in a selected well of a multi-well plate, each well of the multi-well plate being selectable by translating the multi-well plate in a plane substantially perpendicular to the directed excitation light.
12. A reflection light transfer module comprising:
an input mirror for directing an excitation light to a sample;
an output mirror, positioned on a common axis with the input mirror and collecting an emitted light from the sample, the emitted light being emitted by the sample in response to illumination of the sample, wherein the sample is positioned substantially coaxially with the output mirror and the output mirror directs the emitted light away from the sample, and wherein the sample is maintained on a glass slide, the sample being positioned to receive the excitation light by translating the slide in a plane substantially perpendicular to the directed excitation light.
13. The reflection light transfer module of claim 12 , wherein the reflection light transfer module cooperates with microscope optics.
14. The reflection light transfer module of claim 13 , wherein the sample comprises a region of intact biological cells.
15. A reflection light transfer module comprising:
an input mirror for directing an excitation light to a sample;
an output mirror, positioned on a common axis with the input mirror and collecting an emitted light from the sample, the emitted light being emitted by the sample in response to illumination of the sample, wherein the sample is positioned substantially coaxially with the output mirror and the output mirror directs the emitted light away from the sample; and
an output director for directing the emitted light collected by the output mirror to a selected output port.
16. The reflection light transfer module of claim 15 , wherein placement of the output director selects the output port.