Specific salt, anhydrous and crystalline form of a dihydropteridione derivative
The present invention relates to a specific salt of a dihydropteridione derivative, namely the trihydrochloride salt of the compound N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, to its crystallisation in the form of an hydrate with water, to a process for the manufacture thereof, and to the use thereof in a pharmaceutical composition.
1. A process for the manufacture of N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, which has the structural formula (I)
wherein the compound of the formula 15c
is reacted with the compound of the formula 16,
2. The process of claim 1 wherein the compound of formula 16 is prepared by methylation of a compound of formula 8
in the presence of dimethylcarbonate.
3. A process for the manufacture of the trihydrochloride salt of N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, said process comprising the steps of contacting N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, dissolved in a suitable solvent or solvent mixture, with hydrochloric acid or hydrogen chloride gas, dissolved in an organic solvent, and collecting the precipitate formed.
4. A process for the manufacture of the trihydrocliloride salt of N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, which process comprises the step of reacting the compound of the formula 15c
with the compound of the formula 16,
in the presence of hydrochloric acid.
5. The process in accordance with claim 3 or 4 , further comprising the purification of the trihydrochloride salt of N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, wherein said purification comprises the steps of:
(a) suspending the trihydrochioride salt of N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide in a suitable organic solvent;
(b) heating the thus produced suspension of the trihydrochloride salt in organic solvent to reflux;
(c) adding water to the suspension, to obtain a precipitate; and,
(d) collecting the precipitate, washing it and drying it.
6. A process for the manufacture of a hydrated, crystalline form of the trihydrochloride salt of N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide, said process comprising the steps of:
(a) dissolving N-[trans-4-[4-(cyclopropylmethyl)-1-piperazinyl]cyclohexyl]-4-[[(7R)-7-ethyl-5,6,7,8-tetrahydro-5-methyl-8-(1-methylethyl)-6-oxo-2-pteridinyl]amino]-3-methoxy-benzamide in a suitable organic solvent at room temperature or elevated temperature,
(b) adding hydrochloric acid to the solution produced by the preceding step;
(c) cooling the solution produced by the preceding step, to produce a precipitate; and,
(d) collecting the precipitate, washing the precipitate, and drying the precipitate.
7. The process of claim 6 , wherein the hydrated crystal form produced is the trihydrate.