Reagents for arsenic meter
View Patent ↗A formulation for preparing water samples for determining arsenic concentration by colorimetry is provided. The chemical reagents in the formulation may be provided as premeasured and premixed tablets or pills. The chemical reagents include sulfamic acid, an oxidizer, and color reagents for selectively forming molybdenum-based color complexes with arsenates and phosphates. To test for arsenic, the water samples are acidified by addition of sulfamic acid. Sulfamic acid also advantageously reduces arsenic to an arsenite state. Arsenic in the water sample is oxidized or re-oxidized to an arsenate state by addition of the oxidizer. Then, color reagents are used to selectively bind and convert arsenates and phosphates in the water samples into molybdenum-blue color complexes. The light absorbance of the water sample with both arsenates and phosphates bound in molybdenum-blue color complexes is compared to that of a reference acidified specimen in which mostly only the phosphates but not the arsenates are bound and converted. The differential light absorbance of the two specimens is used to arrive at a quantitative value for the arsenic concentration in the water sample.
1. A method for quantitative determination of arsenic concentration in a water sample in the field, wherein the water sample comprises phosphates that have an order of magnitude or more higher concentration than the arsenic concentration, the method comprising:
(a) adding an acid reagent to the water sample to acidify the water and also reduce arsenic;
(b) adding an oxidizing reagent to a test water sample aliquot to oxidize arsenic in the aliquot to an arsenate state;
(c) adding a color reagent to the test water sample aliquot and a reference acidified water sample aliquot, whereby phosphates in the reference aliquot and both phosphates and arsenates in the test aliquot and are converted into color complexes;
(d) using optical probes to measure light absorbance of the color complexes formed in each aliquot; and
(e) using the measured light absorbances for the two aliquots to calculate the arsenic concentration in the water sample.
2. The method of claim 1 wherein adding an acid reagent to the water sample eliminates a separate step of adding a reducing agent that is used in preparation chemistries based on the Johnson and Pilson formulations to reduce arsenic in the water sample to an arsenite state.
3. The method of claim 1 wherein adding an acid reagent to the water sample comprises adding a solid reagent to the water sample.
4. The method of claim 3 wherein the solid reagent comprises sulfamic acid.
5. The method of claim 1 wherein adding an oxidizing agent comprises adding a solid oxidizer.
6. The method of claim 5 wherein the solid oxidizer comprises potassium iodate.
7. The method of claim 1 wherein the color reagent comprises solid reagents.
8. The method of claim 7 wherein the solid reagents comprise sodium molybdate and potassium antimonyl tartrate.
9. The method of claim 8 wherein the solid reagents further comprise ascorbic acid.
10. The method of claim 1 wherein using optical probes comprises using a portable colorimeter.
11. The method of claim 1 wherein the reagents are premeasured and prepared in pill form.
12. The method of claim 1 , wherein
adding an oxidizing reagent comprises adding a solid oxidizing reagent oxidizes arsenic in the water samples to an arsenate state; and
the solid color reagent incorporates the arsenic in the arsenate state in the water sample in a color complex and forms such color complex in a sufficient quantity so that a measurable, differential light absorbance of the color complex with respect to a reference acidified water sample aliquot yields the arsenic concentration in the water sample having phosphates in an order of magnitude or more higher concentration than the arsenic concentration.