Branched peptide amphiphiles, related epitope compounds and self assembled structures thereof
Branched peptide amphiphilic compounds incorporating one or residues providing a pendant amino group for coupling one or more epitope sequences thereto, such compounds and related compositions for enhanced epitope presentation.
1. A non-linear peptide amphiphile compound comprising a peptide component having the formula KXXXAAAK (SEQ ID NO: 10), wherein X is G or L, which peptide component further has at least one pendant lysine residue coupled to a component non-linear with said peptide component, wherein the component non-linear with said peptide component is selected from IKVAV (SEQ ID NO: 3), YIGSR (SEQ ID NO: 4), RGDS (SEQ ID NO: 1), and PHSRN (SEQ ID NO: 2), wherein said peptide component is coupled to a hydrophobic component, and wherein the hydrophobic component is an alkyl moiety ranging from C 6 -C 22 .
2. The compound of claim 1 wherein X is L.
3. The compound of claim 2 wherein IKVAV (SEQ ID NO: 3) and YIGSR (SEQ ID NO: 4) are non-linear to said peptide component.
4. The compound of claim 3 wherein said compound has the following formula:
IKVAVK
KLLLAAAKC 16
YIGSRK.
5. The compound of claim 1 wherein each of said two lysine residues is coupled to one of said epitope sequences.
6. The compound of claim 5 wherein at least one of said sequences is coupled to a spectroscopic probe component.
7. The compound of claim 1 assembled into a micellar configuration.
8. A system comprising a non-linear peptide amphiphile compound comprising a peptide component, a hydrophobic component coupled to said peptide component at its N-terminus, said peptide component comprising a peptide of the formula KXXXAAAK (SEQ ID NO: 11), wherein X is L or K, further comprising at least one pendant lysine residue which is coupled to a sequence selected from IKVAV (SEQ ID NO: 3), YIGSR (SEQ ID NO: 4), RGDS (SEQ ID NO: 1), and PHSRN (SEQ ID NO: 2), wherein the hydrophobic component is an alkyl moiety ranging from C 6 -C 22 , and wherein said compound is in a fluid medium.
9. The system of claim 8 wherein X is L.
10. The system of claim 9 wherein said compound comprises IKVAV (SEQ ID NO: 3) and YIGSR (SEQ ID NO: 4).
11. The system of claim 8 wherein the compound has the following formula:
IKVAVK
KLLLAAAKC 16
YIGSRK.
12. The system of claim 8 wherein said medium is at a physiological pH, said system comprising an assembly of said compounds.
13. A method of using a pendant lysine residue of a peptide amphiphile to present a biologically active epitope sequence, said method comprising: providing a plurality of peptide amphiphile compounds, each of said compounds comprising a peptide component having the formula KXXXAAAK (SEQ ID NO:10), wherein X is G or L, and a hydrophobic component selected from an alkyl moiety ranging from C 6 -C 22 , at least one of said compounds comprising at least one pendant lysine residue; and coupling a biologically active epitope sequence selected from IKVAV (SEQ ID NO:3), YIGSR (SEQ ID NO:4), RGDS (SEQ ID NO:1), PHSRN (SEQ ID NO:2) to said pendant lysine residue, wherein said epitope sequence is non-linear to said peptide component.
14. The method of claim 13 wherein said epitope sequence further includes the recognition product of a phage display process.
15. The method of claim 13 wherein the N-terminus of said peptide component comprises two lysine residues.
16. The method of claim 15 wherein each of said lysine residues is coupled to an epitope sequence selected from RDGS (SEQ ID NO: 1), PHSRN (SEQ ID NO: 2), IKVAV (SEQ ID NO: 3), YIGSR (SEQ ID NO: 4) and the recognition product of a phage display process.
17. The method of claim 13 wherein said compounds are in an aqueous medium, and the pH of said medium is adjusted to assemble said compounds in a cylindrical micelle.
18. The method of claim 13 wherein at least one of said compounds comprises a peptide component comprising at least two lysine residues.
19. The method of claim 18 wherein each of said residues is coupled to an epitope sequence selected from RGDS (SEQ ID NO: 1), PHSRN (SEQ ID NO: 2), IKVAV (SEQ ID NO: 3), YIGSR (SEQ ID NO: 4) and the recognition product of a phage display process.
20. The method of claim 19 wherein one of said residues is coupled to an RGDS (SEQ ID NO: 1) epitope and one of said residues is coupled to a PHSRN (SEQ ID NO: 2) epitope.
21. The method of claim 20 wherein said compounds are in an aqueous medium and assembled using a reagent in said medium.