Psoriasin expression by breast epithelial cells
The invention features methods of diagnosing high grade ductal carcinoma in situ (DCIS) These methods involve measuring: (1) the level of HID-5 in a body fluid (e.g., blood or urine) of a subject suspected of having, or at risk of having, high grade DCIS; or (2) the level of HID-5 gene expression in breast tissue from a subject suspected of having, or at risk of having, high grade DCIS. The invention also embodies a method of inhibiting expression of HID-5 protein in DCIS cells and methods of treating a subject suspected of having, or at risk of having, high grade DCIS.
1. A method of diagnosis, the method comprising:
(a) identifying a subject suspected of having or at risk of having high-grade ductal carcinoma in situ (DCIS); and
(b) measuring the level of psoriasin in a sample of a body fluid, a lavage, or an aspirate from the subject,
wherein an elevated level of psoriasin in the sample, compared to a control level of psoriasin, is an indication that the subject has high grade DCIS.
2. The method of claim 1 , wherein the body fluid is blood.
3. The method of claim 1 , wherein the body fluid is urine.
4. The method of claim 1 , wherein the lavage is a breast duct lavage.
5. The method of claim 1 , wherein the aspirate is a nipple aspirate.