Angiotensin converting enzyme homolog and uses therefor
The present invention relates to the discovery of novel genes encoding an angiotensin converting enzyme, Angiotensin Converting Enzyme-2 (ACE-2). The invention provides therapeutics, prognostic and diagnostics methods for treating blood pressure related disorders as well as various types of allergic conditions, among others. Also disclosed are screening assays for identifying compounds for treating and preventing these conditions.
1. A method for identifying an ACE-2 antagonist, comprising
(i) combining an ACE-2 polypeptide, an ACE-2 binding partner and a test compound under conditions wherein, but for the test compound, the ACE-2 polypeptide and ACE-2 binding partner form a complex; and
(ii) detecting the formation of an ACE-2 polypeptide/ACE-2 binding partner complex, wherein a reduction in the formation of an ACE-2 polypeptide/ACE-2 binding partner complex in the presence of a test compound relative to the absence of the test compound indicates that the test compound is an ACE-2 antagonist.
2. The method of claim 1 , wherein the ACE-2 binding partner is a target peptide or analog thereof.
3. The method of claim 2 , wherein the binding partner is angiotensin I or a portion thereof or an analog thereof sufficient for binding to an ACE-2 polypeptide.
4. The method of claim 2 , wherein the binding partner is angiotensin I or a portion thereof or an analog thereof sufficient for binding to, and being hydrolyzed by, an ACE-2 polypeptide.
5. The method of claim 2 , wherein the binding partner is kinetensin or a portion thereof or an analog thereof sufficient for binding to an ACE-2 polypeptide.
6. The method of claim 2 , wherein the binding partner is kinetensin or a portion thereof or an analog thereof sufficient for binding to, and being hydrolyzed by, an ACE-2 polypeptide.