Methods for the preparation and formulation of magnesium valproate hydrate
The present invention relates to methods for preparing magnesium valproate hydrate and administering this compound to a subject in need of treatment with valproate. Pharmaceutical compositions are also provided that are useful therapies for the treatment of neurological, immunological, and viral-mediated disorders in warm-blooded mammals.
1. A magnesium valproate hydrate composition, having the molecular formula C 16 H 30 O 4 Mg·x H 2 O, where x is 0.7, and a weight percent valproate content of about 85% up to about 91%.
2. The composition of claim 1 , wherein said magnesium valproate hydrate has a polymorphic structure having both crystalline and amorphous structural properties.
3. A method of obtaining magnesium valproate hydrate from an alcohol solution containing magnesium valproate comprising adding to the alcohol solution a volume of a polar, non-oxygen-containing organic solvent having a boiling point of 130° C. or lower, the volume of said solvent being sufficient to reduce the solubility of the magnesium valproate in the resulting reaction mixture to less than 0.01 g/mL, and isolating the magnesium valproate hydrate from the resulting reaction mixture.
4. The method of claim 3 , wherein the alcohol is selected from a group consisting of methanol, ethanol, propanol, 2-propanol, and a mixture thereof and the polar, non-oxygen-containing organic solvent is selected from a group consisting of acetonitrile, propionitrile, butyronitrile, and isobutyronitrile.
5. A method of preparing magnesium valproate hydrate comprising reacting sodium valproate and a magnesium halide selected from a group consisting of magnesium chloride, magnesium bromide, and magnesium iodide in stoichiometric proportions in an alcoholic solvent selected from a group consisting of ethanol and methanol, removing the sodium halide, and recovering magnesium valproate hydrate from the reaction mixture.
6. A method of preparing magnesium valproate hydrate comprising reacting valproic acid and magnesium methoxide in stoichiometric proportions in an alcoholic solvent selected from a group consisting of methanol, ethanol, propanol, 2-propanol, and a mixture thereof, adding to the alcohol solution a volume of a polar, non-oxygen-containing organic solvent having a boiling point of 130° C or lower, the volume of said solvent being sufficient to reduce the solubility of the magnesium valproate in the resulting reaction mixture to less than 0.01 g/mL, and recovering magnesium valproate hydrate from the reaction mixture.
7. A method of preparing magnesium valproate hydrate comprising reacting magnesium ethoxide and valproic acid in 1% 10% stoichiometric excess in an alcoholic solvent selected from a group consisting of methanol, ethanol, propanol, 2-propanol, and a mixture thereof, adding to the alcohol solution a volume of a polar, non-oxygen-containing organic solvent having a boiling point of 130° C or lower, the volume of said solvent being sufficient to reduce the solubility of the magnesium valproate in the resulting reaction mixture to less than 0.01 g/mL, and recovering magnesium valproate hydrate from the reaction mixture.
8. The method of claim 5 , 6 , or 7 , wherein magnesium valproate hydrate is recovered from the reaction mixture by adding a volume of acetonitrile sufficient to cause precipitation of said magnesium valproate hydrate.
9. The method of claim 8 , wherein upon adding acetonitrile, the solubility of said magnesium valoroate hydrate is less than about 0.01 g/mL.
10. A method of preparing an aqueous solution of magnesium valproate having a concentration of valproate in the range from about 1 mg/mL to about 150 mg/mL comprising dissolving a known mass of magnesium valproate hydrate in a volume of water sufficient to provide a solution having a valproate concentration in the range from about 1 mg/mL to about 150 mg/mL.
11. A method of preparing an aqueous solution of magnesium valproate having a concentration of valproate in the range from about 1 mg/mL to about 150 mg/mL comprising dissolving a known mass of magnesium valproate hydrate in a volume of a solvent selected from a group consisting of 1-10% dextrose in water, 1-10% poly(ethylene glycol) in water, 1-10% propylene glycol in water, and 1-10% glycerol in water sufficient to provide a solution having a valproate concentration in the range from about 1 mg/mL to about 150 mg/mL.
12. A method of preparing a pharmaceutical formulation of magnesium valproate having a concentration of valproate in the range from about 1 mg/mL to about 150 mg/mL comprising dissolving a known mass of magnesium valproate hydrate in a volume of a solvent selected from a group consisting of water, 1-10% dextrose in water, 1-10% poly(ethylene glycol) in water, 1-10% propylene glycol in water, and 1-10% glycerol in water sufficient to provide a solution having a valproate concentration in the range from about 1 mg/mL to about 150 mg/mL, and optionally, sterilizing the formulation.
13. A method of obtaining magnesium valproate hydrate from a solution of magnesium valproate in a solvent selected from a group consisting of methanol, ethanol, propanol, 2-propanol, and a mixture thereof comprising adding to the solution a volume of acetonitrile sufficient to reduce the solubility of the magnesium valproate in the resulting reaction mixture to less than about 0.01 g/mL, and recovering magnesium valproate hydrate from the reaction mixture.
14. A method of preparing magnesium valproate hydrate comprising reacting sodium valproate and a magnesium halide selected from a group consisting of magnesium chloride, magnesium bromide, and magnesium iodide in stoichiometric proportions in an alcoholic solvent selected from a group consisting of ethanol and methanol, removing the sodium halide, and recovering magnesium valproate hydrate from the reaction mixture by adding a polar, non-oxygen-containing organic solvent selected from a group consisting of acetonitrile, propionitrile, butyronitrile, and isobutyronitrile in a volume sufficient to reduce the solubility of magnesium valproate in the resulting reaction mixture to less than about 10 mg/mL. and recovering magnesium valproate hydrate from the reaction mixture.
15. A method of preparing magnesium valproate hydrate comprising reacting valproic acid and magnesium methoxide in stoichiometric proportions in an alcoholic solvent selected from a group consisting of methanol, ethanol, propanol, 2-propanol, and a mixture thereof, and recovering magnesium valproate hydrate from the reaction mixture by adding a polar, non-oxygen-containing organic solvent selected from a group consisting of acetonitrile, propionitrile, butyronitrile, and isobutyronitnie in a volume sufficient to reduce the solubility of magnesium valproate in the resulting reaction mixture to less than about 10 mg/mL, and recovering magnesium valproate hydrate from the resulting reaction mixture.
16. A method of preparing magnesium valproate hydrate comprising reacting valproic acid in 1%-10% stoichiometric excess and magnesium ethoxide in an alcoholic solvent selected from a group consisting of methanol, ethanol, propanol, 2-propanol, and a mixture thereof, and recovering magnesium valproate hydrate from the reaction mixture by adding a polar, non-oxygen-containing organic solvent selected from a group consisting of acetonitrile, propionitrile, butyronitrile, and isobutyronitrile in a volume sufficient to reduce the solubility of magnesium valproate in the resulting reaction mixture to less than about 10 mg/mL, and recovering magnesium valproate hydrate from the resulting reaction mixture.