Polynucleotide encoding an intracellular estradiol binding protein
Described herein is a novel intracellular estradiol binding protein (“IEBP”), as well as a polynucleotide encoding this protein and various cells and cell lines producing and/or overexpressing it. IEBP is believed to play a role in the modulation of estrogen signaling and in the physiological resistance to the same. Abnormally elevated or decreased levels of IEBP may thus be a component of the etiology of diseases generally correlated with estrogen signaling, such as, by way of example, breast cancer and osteoporosis. Various embodiments of the present invention are believed to provide important tools for developing treatments for these conditions, such as, for example, by providing means for screening therapeutic compounds and identifying a genetic target for therapy.
1. An isolated nucleic acid encoding an intracellular estradiol binding protein (IEBP), selected from the group consisting of:
(a) DNA encoding the amino acid sequence set forth in SEQ ID NO:1,
(b) DNA degenerate with respect to either (a) or (b), wherein said DNA encodes biologically active IEBP, and
(c) DNA fully complementary to any of (a) or (b).
2. The isolated nucleic acid of claim 1 , wherein said isolated nucleic acid is selected from the group consisting of genomic DNA and cDNA.
3. The isolated nucleic acid of claim 1 , comprising a nucleic acid that further comprises substantially the same nucleic acid sequence as set forth in SEQ ID NO:2.
4. The isolated nucleic acid of claim 1 , comprising a nucleic acid that further comprises substantially the same nucleic acid sequence as set forth in SEQ ID NO:3.
5. An isolated vector comprising the polynucleotide of claim 1 .
6. An isolated host cell comprising the vector of claim 5 .
7. An isolated recombinant host cell comprising the isolated nucleic acid of claim 1 .
8. The recombinant host cell of claim 7 , wherein said host cell is selected from the group consisting of a B95-8 cell, a 6299 breast cell, and an E. coli cell.
9. The recombinant host cell of claim 7 , wherein said host cell overexpresses IEBP.
10. An isolated composition, comprising the host cell of claim 7 , and a carrier.
11. The composition of claim 10 , wherein said carrier is selected from the group consisting of phosphate buffered saline (PBS) solution, water, emulsions, and wetting agents.