Synthetic HLA binding peptide analogues and uses thereof
View Patent ↗The present invention provides synthetic peptides comprising at least analogues of a native peptide that specifically bind to HLA A0201 or HLA A0301 molecules on a cell characteristic of a pathophysiologic state, such as a cancer cell, in a mammal. Also provided are pharmaceutical compositions and immunogenic compositions comprising at least the peptide analogue segments or a DNA encoding the same. Also provided are methods of using the synthetic peptides and immunogenic compositions to induce a heteroclitic immune response or to treat a cancer.
1. A synthetic peptide, comprising:
a sequence of amino acids containing at least a segment that is an analogue of a native peptide that specifically binds to HLA A0201 or HLA A0301 molecules on a cell characteristic of a pathophysiologic state in a mammal, wherein said segment comprises YMFPNAPYL (SEQ ID NO:18).
2. The synthetic peptide of claim 1 , further comprising:
an immunogenic carrier linked thereto.
3. The synthetic peptide of claim 2 , wherein said immunogenic carrier is a protein, a peptide or an antigen-presenting cell.
4. The synthetic peptide of claim 3 , wherein said protein or peptide is keyhole limpet hemocyanin, an albumin or a polyamino acid.
5. The synthetic peptide of claim 3 , wherein said antigen presenting cell is a dendritic cell.
6. A pharmaceutical composition, comprising:
a therapeutically effective amount of the synthetic peptide of claim 1 ; and
a pharmaceutically acceptable carrier.
7. An immunogenic composition comprising an immunogenically effective amount of the synthetic peptide of claim 1 and a pharmaceutically acceptable carrier, adjuvant or diluent or a combination thereof.
8. The immunogenic composition of claim 7 , wherein said immunogenic carrier is a protein, a peptide or an antigen presenting cell linked to said synthetic peptide.
9. The immunogenic composition of claim 8 , wherein said protein or peptide is keyhole limpet hemocyanin, an albumin or a polyamino acid.
10. The immunogenic composition of claim 8 , wherein said antigen presenting cell is a dendritic cell.