Human RNase III and compositions and uses thereof
The present invention provides polynucleotides encoding human RNase III and polypeptides encoded thereby. Methods of using said polynucleotides and polypeptides are also provided.
1. A method of increasing cleavage of a target RNA in a mammalian cell comprising increasing the amount of a polypeptide in the cell, wherein the polypeptide comprises amino acids 906-1374 of the amino acid sequence recited as SEQ ID NO: 2 and wherein the RNA target comprises a double helical structure; and thereby increasing cleavage of the target RNA in the mammalian cell.
2. The method of claim 1 , wherein the increasing the amount of the polypeptide comprises overexpression of the polypeptide.
3. The method of claim 1 , wherein the polypeptide consists of the amino acid sequence recited as SEQ ID NO: 2.
4. The method of claim 1 , wherein the cell is a human cell.
5. The method of claim 4 , wherein the increasing the amount of the polypeptide comprises overexpression of the polypeptide.
6. The method of claim 4 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO: 2.