Incubator and culture device
Contamination by dust particles, bacteria and so forth in each processing step can be reduced by a simple constitution. A culture vessel is used in which a medium vessel in which a medium is sealed and a waste medium vessel capable of housing the fluid in a main culture vessel are connected to the main culture vessel by connecting lines capable of restricting the flow of fluid between them in a state in which they are sealed from the outside. A culture apparatus is composed in which the inner volume of each vessel is variable, and is provided with a case provided with indentations for housing each vessel, and a pressing means that contracts the vessels housed in the indentations by applying external pressure.
1. A culture apparatus comprising:
a culture vessel comprising:
a main culture vessel,
at least one medium vessel in which a medium is sealed, and
at least one waste medium vessel capable of receiving and housing fluid from the main culture vessel,
a case provided wit indentations for housing each vessel that composes the culture vessel,
connecting lines being capable of restricting the flow of fluid between the vessels only in one direction, being made of a flexible material, passing through a connecting line pathway disposed in the case, and comprising:
a first connecting line that provides fluid connection only in the direction from the medium vessel to the main culture vessel, and
a second connecting line that provides fluid connection only in the direction from the main culture vessel to the waste medium vessel,
controlling device including cheek valves and allowing a fluid within the vessels that compose the culture vessel to flow into the connecting lines provided in said vessels, and
a pressing device that contracts each vessel housed in the indentations by applying external pressure,
wherein
said main culture vessel and said medium vessel being sealed from the outside by the connecting lines,
each vessel that composes the culture vessel has a variable inner volume, and
the flow of fluid within the connecting lines is restricted by clamping the lines by the valve disposed in the connecting line pathways in the radial direction.
2. A culture apparatus according to claim 1 , wherein a centrifuge is provided that rotates the case with the culture vessel inside, and the indentations that house the main culture vessels are disposed at intervals from the axis of rotation.
3. A culture apparatus according to claim 1 , wherein an occluded connecting line having an occluded end is provided on the main culture vessel, the occluded connecting line is made of a material that can be sealed or fused with heat, the case is provided with a centrifuge that rotates the case with culture vessels inside, and the occluded connecting line is disposed outward in the radial direction of the main reaction vessel centered about the center of the axis of rotation in the state in which the main culture vessel is housed in indentations of the case.