Artificial cpg single-stranded oligodeoxynucleotide and antiviral use thereof
The present invention provides a series of artificial CpG-containing single-stranded oligodeoxynucleotides (ODNs), each of which is consisted of single-stranded oligodeoxynucleotide DNA molecule containing one or more CpG(s), wherein said ODNs can stimulate human peripheral blood mononuclear cell (PBMC) to produce antiviral substances. These ODNs can protect the cells against the attack from virus, wherein said virus is preferably selected from the group consisted of influenza virus and single-stranded positive strand RNA virus such as SARS virus, hepatitis C virus, dengue virus and Japanese encephalitis virus. Moreover, the antiviral use of artificial CpG ODNs and its use for treating and preventing viral infection are also provided.
1. An artificial CpG-containing single-stranded oligodeoxynucleotide, which consists of a single-stranded oligonucleotide DNA molecule containing one or more CpG(s) which has a sequence as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or SEQ ID NO: 5.
2. The oligonucleotide according to claim 1 , which has a function of inducing the production of antiviral substances from cell.
3. The oligonucleotide according to claim 2 , wherein the antiviral substances are against a single-stranded positive strand RNA virus or influenza virus.
4. The oligonucleotide according to claim 3 , wherein said single-stranded positive strand RNA virus is a variant of coronavirus or flaviviridae virus.
5. The oligonucleotide according to claim 4 , wherein said variant of coronavirus is SARS virus.
6. The oligonucleotide according to claim 4 , wherein said flaviviridae virus is hepatitis C virus, dengue virus or Japanese encephalitis virus.
7. The oligonucleotide according to claim 1 , which has a non-sulfurized, partially sulfurized or completely sulfurized phosphodiester bond.
8. A composition comprising the oligonucleotide according to claim 1 .
9. The composition according to claim 8 , further comprising other antiviral drugs or vaccines.
10. A method for inducing the production of antiviral substances from cell comprising treating said cell with the oligonucleotide of claim 1 .
11. The method of claim 10 , wherein the antiviral substances are against single-stranded positive strand RNA virus or influenza virus.
12. The method of claim 11 , wherein said single-stranded positive strand RNA virus is a variant of coronavirus or flaviviridae virus.
13. The method of claim 12 , wherein said variant of coronavirus is SARS virus.
14. The method of claim 12 , wherein said flaviviridae virus is hepatitis C virus, dengue virus or Japanese encephalitis virus.