Proteins having a flavonoid 5-O-glycosyltransferase activity (5GT)
View Patent ↗The present invention provides a gene that codes for a protein, wherein the protein is an anthocyanin 5-O-glucosyltransferase, which is an enzyme that transfers a glycoside to the 5 position of a flavonoid. The disclosed anthocyanin 5-O-glucosyltransferases were isolated from Perilla fruescens, Verbena hybrida, Torenia hybrida , and Petunia hybrida . Also disclosed are genes that code for a protein having a modified amino acid sequence relative to the above amino acid sequence and having activity that transfers a glycoside to the 5 position of a flavonoid, and a process for producing the above protein using said gene. This gene can be used to artificially alter the color of plants.
1. An isolated protein having an activity that transfers a glycoside to the 5 position of a flavonoid, wherein said protein comprises a sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, and 8.
2. The isolated protein of claim 1 , wherein said protein comprises the sequence of SEQ ID NO: 2.
3. The isolated protein of claim 1 , wherein said protein comprises the sequence of SEQ ID NO: 4.
4. The isolated protein of claim 1 , wherein said protein comprises the sequence of SEQ ID NO: 6.
5. The isolated protein of claim 1 , wherein said protein comprises the sequence of SEQ ID NO: 8.