Nucleic acids encoding rat hyaluronan receptor for endocytosis
View Patent ↗A purified nucleic acid segment encoding a functionally active hyaluronan receptor for endocytosis (HARE) or an active peptide fragment thereof, and methods for producing functionally active HARE or an active peptide fragment thereof therefrom, wherein the functionally active HARE or an active peptide fragment thereof is able to specifically bind HA, chondroitin and chondroitin sulfate.
1. A purified nucleic acid segment comprising a coding region encoding a functionally active Hyaluronic Acid Receptor for Endocytosis (HARE), wherein the purified nucleic acid segment comprises the nucleotide sequence in accordance with SEQ ID NO:1.
2. A recombinant vector selected from the group consisting of a plasmid, cosmid, phage, or virus vector and wherein the recombinant vector further comprises the purified nucleic acid segment of claim 1 .
3. The recombinant vector of claim 2 , wherein the plasmid is an expression vector.
4. The recombinant vector of claim 3 , wherein the expression vector comprises a promoter operatively linked to the HARE coding region.
5. A recombinant host cell, comprising the recombinant vector of claim 2 .
6. The recombinant host cell of claim 5 , wherein the host cell is a eucaryotic cell.
7. The recombinant host cell of claim 5 , wherein the recombinant host cell produces a functionally active HARE which specifically binds and endocytoses at least one of HA, chondroitin, and chondroitin sulfate.
8. The recombinant host cell of claim 5 , wherein the purified nucleic acid segment is integrated into a chromosome of the recombinant host cell.
9. A method of producing a functionally active Hyaluronic Acid Receptor for Endocytosis (HARE) wherein the HARE is able to specifically bind at least one of HA, chondroitin, and chondroitin sulfate, the method comprising the steps of:
providing the recombinant host cell of claim 5 ;
culturing the recombinant host cell under conditions that allow for expression of the recombinant DNA segment encoding a functionally active HARE, thereby producing functionally active HARE; and
separating and purifying the functionally active HARE from the recombinant host cell.
10. A purified nucleic acid segment comprising a coding region encoding a functionally active Hyaluronic Acid Receptor for Endocytosis (HARE), wherein the purified nucleic acid segment comprises a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2.
11. A recombinant vector selected from the group consisting of a plasmid, cosmid, phage, or virus vector and wherein the recombinant vector further comprises the purified nucleic acid segment of claim 10 .
12. The recombinant vector of claim 11 , wherein the plasmid is an expression vector.
13. The recombinant vector of claim 12 , wherein the expression vector comprises a promoter operatively linked to the HARE coding region.
14. A recombinant host cell, comprising the recombinant vector of claim 11 .
15. The recombinant host cell of claim 14 , wherein the host cell is a eucaryotic cell.
16. The recombinant host cell of claim 14 , wherein the recombinant host cell produces a functionally active HARE which specifically binds and endocytoses at least one of HA, chondroitin, and chondroitin sulfate.
17. The recombinant host cell of claim 14 , wherein the purified nucleic acid segment is integrated into a chromosome of the recombinant host cell.
18. A method of producing a functionally active Hyaluronic Acid Receptor for Endocytosis (HARE) wherein the HARE is able to specifically bind at least one of HA, chondroitin, and chondroitin sulfate, the method comprising the steps of:
providing the recombinant host cell of claim 14 ;
culturing the recombinant host cell under conditions that allow for expression of the recombinant DNA segment encoding a functionally active HARE, thereby producing functionally active HARE; and
separating and purifying the functionally active HARE from the recombinant host cell.