Smart disposable plastic lab-on-a-chip for point-of-care testing
View Patent ↗Disclosed herein is a fully-integrated, disposable biochip for point-of-care testing of clinically relevant parameters. Specifically, in accordance with an embodiment of the present invention, the biochip is designed for POCT (point-of-care-testing) of an array of metabolic parameters including partial pressure of oxygen, Glucose, and Lactate concentration from venous blood samples. The biochip is fabricated on a low-cost plastic substrate using mass manufacturing compatible fabrication processes. Furthermore, the biochip contains a fully-integrated metallic micro-needle for blood sampling. The biochip also uses smart passive microfluidics in conjunction with low-power functional on-chip pressure generators for microfluidic sequencing. The design, configuration, assembly and operation of the biochip are ideally suited for a disposable biochip specifically targeted towards POCT applications.
1. A disposable, integrated biochip for clinical diagnostic applications, said biochip comprising:
a. at least one micro-sensor for sensing at least one relevant parameter of said sampled fluid;
b. micro-calibration system connected to said at least one micro-sensor to calibrate said biochip comprising:
i. a metallic calibration solution pouch containing a calibration buffer of at least one known concentration of at least one analyte;
ii. at least one micro-fluidic channel, connecting to said calibration solution pouch, to carry said calibration buffer to said at least one micro-sensor when said calibration solution pouch is ruptured; and
iii. at least one waste reservoir to accept said calibration buffer after said biochip is calibrated;
c. a micro-needle for sampling a fluid from a subject; and
d. a passive micro-fluidic control system, connecting said micro-needle to said at least one micro-sensor, to accept said sampled fluid from said micro-needle and to facilitate movement of said sampled fluid to said at least one micro-sensor.
2. The biochip of claim 1 further comprising a micro-propulsion system connecting to said passive micro-fluidic control system to move said sampled fluid through said passive micro-fluidic control system.
3. The biochip of claim 2 wherein said at least one micro-sensor, said passive micro-fluidic control system, said micro-propulsion system, and said micro-calibration system are collectively fabricated on at least two plastic substrates that are integrated together within said biochip.
4. The biochip of claim 1 wherein said micro-needle is metallic.
5. The biochip of claim 1 wherein said at least one micro-sensor comprises at least one of an electro-chemical oxygen biosensor, an electrochemical glucose biosensor, an electro-chemical lactate biosensor, a pH biosensor, and a hematocrit biosensor.
6. The biochip of claim 1 wherein said passive micro-fluidic system comprises: a micro-dispenser reservoir; at least one passive micro-valve; at least one micro-sensor reservoir connecting to said at least one micro-sensor via at least one semi-permeable membrane of said at least one micro-sensor; and at least two micro-fluidic channels connecting together said micro-dispenser reservoir, said at least one passive micro-valve, and said at least one micro-sensor reservoir.
7. The biochip of claim 2 wherein said micro-propulsion system comprises: at least one micro-chamber containing a pressurized gas and being connected to said passive micro-fluidic control system; and at least one micro-heater positioned next to and being in contact with a thermo-plastic membrane of said micro-chamber to release said pressurized gas into an air inlet of said passive micro-fluidic control system upon melting of said thermo-plastic membrane by said micro-heater when an electrical pulse is applied to said micro-heater.
8. The biochip of claim 2 wherein said micro-propulsion system comprises: at least one micro-heater; and at least one solid chemical-propellant positioned on said at least one micro-heater to release an inert gas into an air inlet of said passive micro-fluidic control system upon activation of said at least one solid chemical propellant by said micro-heater when an electrical pulse is applied to said micro-heater.
9. The biochip of claim 3 wherein said at least two plastic substrates comprises at least one of cyclic olefin copolymer, polyimide, polymethylmethaacrylate, PDMS, polyethylene, and polycarbonate.
10. The biochip of claim 5 wherein said oxygen biosensor comprises: a semi-permeable membrane that is permeable to at least oxygen molecules; a solid, gel-based layer of electrolyte solution being in contact with said semi-permeable membrane; and at least two electrodes being in contact with said solid, gel-based layer of electrolyte solution.
11. The biochip of claim 5 wherein said glucose biosensor comprises: a first semi-permeable membrane that is permeable to at least glucose molecules; a glucose oxidase enzyme layer being in contact with said first semi-permeable membrane to oxidize said glucose molecules into at least oxygen molecules; a second semi-permeable membrane being in contact with said glucose oxidase enzyme layer and being permeable to at least said oxygen molecules; a solid, gel-based layer of electrolyte solution being in contact with said second semi-permeable membrane; and at least two electrodes being in contact with said solid, gel-based layer of electrolyte solution.
12. The biochip of claim 5 wherein said lactate biosensor comprises: a first semi-permeable membrane that is permeable to at least lactate molecules; a lactate oxidase enzyme layer being in contact with said first semi-permeable membrane to oxidize said lactate molecules into at least oxygen molecules; a second semi-permeable membrane being in contact with said lactate oxidase enzyme layer and being permeable to at least said oxygen molecules; a solid, gel-based layer of electrolyte solution being in contact with said second semi-permeable membrane; and at least two electrodes being in contact with said solid, gel-based layer of electrolyte solution.
13. The biochip of claim 1 further comprising at least two conductive traces connected to said at least one micro-sensor for monitoring an electric current generated by said micro-sensor.
14. The biochip of claim 1 wherein said at least one relevant parameter comprises at least one of partial oxygen concentration, glucose level, lactate level, pH, and hematocrit.