Compositions and methods for intracellular delivery of biotinylated cargo
The invention provides compositions for intracellular delivery of biotinylated cargo. The compositions comprise a complex formed between (a) a fusion protein comprising a protein transduction domain linked to streptavidin and (b) a biotinylated cargo for intracellular delivery. In some embodiments, the protein transduction domain comprises the protein transduction domain of the Human Immunodeficiency Virus type 1 (HIV-1) TAT protein. The complex may further comprise a biotinylated endosomal releasing polymer, such as a poly(propylacrylic acid) polymer. The invention also provides methods for obtaining intracellular delivery of biotinylated cargo and methods for obtaining cytoplasmic delivery of biotinylated cargo.
1. A composition, comprising a complex formed between (a) a fusion protein comprising a protein transduction domain linked to streptavidin (b) a biotinylated cargo for intracellular delivery and (c) a (propylacrylic acid)-containing polymer.
2. The composition of claim 1 , wherein the protein transduction domain comprises the protein transduction domain of the Human Immunodeficiency Virus type 1 (HIV-1) TAT protein.
3. The method of claim 1 , wherein the (propylacrylic acid)-containing polymer is poly(propylacrylic acid).
4. The composition of claim 1 , wherein the (propylacrylic acid)-containing polymer is biotinylated.
5. A method for obtaining intracellular delivery of biotinylated cargo, comprising the step of exposing cells to a complex comprising (a) a fusion protein comprising a protein transduction domain linked to streptavidin to obtain intracellular delivery of the biotinylated cargo (b) a biotinylated cargo and (c) a (propylacrylic acid)-containing polymer.
6. The method of claim 5 , wherein the protein transduction domain comprises the protein transduction domain of the HIV-1 TAT protein.
7. The method of claim 5 , wherein the (propylacrylic acid)-containing polymer is biotinylated.
8. A method for obtaining cytoplasmic delivery of biotinylated cargo, comprising the step of exposing cells to a complex comprising (a) a fusion protein comprising a protein transduction domain linked to streptavidin to obtain cytoplasmic delivery of the biotinylated cargo, (b) a biotinylated cargo, and (c) a biotinylated (propylacrylic acid)-containing polymer.
9. The method of claim 8 , wherein the (propylacrylic acid)-containing polymer is poly(propylacrylic acid).
10. The method of claim 9 , wherein the protein transduction domain comprises the protein transduction domain of the HIV-1 TAT protein.