Tet repressor-based transcriptional regulatory proteins
The present invention provides a panel of transcriptional activator fusion proteins which comprises both tetracycline controlled transactivator proteins and reverse tetracycline transactivator proteins. These transactivators have novel phenotypes such as altered basal transcriptional activity in the absence of doxycycline, altered induced transcriptional activity in the presence of doxycycline, or differential induction by tetracycline and analogs of tetracycline.
1. An isolated polynucleotide selected from the group consisting of:
(a) a polynucleotide having the nucleic acid sequence as shown in SEQ ID No:5;
(b) a polynucleotide encoding a first polypeptide, wherein the first polypeptide differs from the polypeptide encoded by SEQ ID NO: 5 by a single amino acid, wherein the amino acid difference is S12G;
(c) a polynucleotide having a nucleic acid sequence which differs from the nucleic acid sequence of (a) due to the degeneracy of the genetic code;
wherein the polypeptide encoded by each of the polynucleotides (a), (b), and (c) is capable of inducing transcriptional activity in the presence of tetracycline, tetracycline derivatives, or tetracycline analogs in a host cell.
2. The polynucleotide of claim 1 , wherein the host cell is a mammalian cell.
3. The polynucleotide of claim 1 , wherein the tetracycline analog is doxycycline.
4. The polynucleotide of claim 1 , wherein the polypeptide encoded by the polynucleotide mediates an increased induced transcriptional activity in the presence of tetracycline, tetracycline derivatives, or tetracycline analogs or a decreased basal transcriptional activity in the absence of tetracycline, tetracycline derivatives, or tetracycline analogs compared to the rtTA polypeptide encoded by the nucleic acid sequence shown in FIG. 8 , wherein the tetracycline repressor is fused to three minimal activation domains.
5. A vector comprising the polynucleotide of claim 1 .
6. The vector of claim 5 , wherein the vector is an expression vector.
7. The vector of claim 5 , wherein the vector is selected from the group consisting of: pCM190GFP+, pUHD15-1, pREP9, pUHD, and baculovirus expression vectors.
8. An isolated host cell comprising the vector of claim 5 .
9. The host cell of claim 8 , wherein the host cell is selected from the group consisting of: a plant cell, an insect cell, a fungal cell, a bacterial cell or a mammalian cell.
10. A method for producing a protein encoded by the polynucleotide of claim 1 comprising:
inserting the polynucleotide of claim 1 into an expression vector;
transforming or transfecting an isolated host cell;
incubating the host cell under conditions conducive for protein expression;
producing the protein encoded by the polynucleotide of claim 1 .
11. The method of claim 10 , wherein the expression vector is selected from the group consisting of: pCM190GFP+, pUHD15-1, pREP9, pUHD, and baculovirus expression vectors.
12. The method of claim 10 , wherein the isolated host cell is selected from the group consisting of: a plant cell, an insect cell, a fungal cell, a bacterial cell or a mammalian cell.
13. The method of claim 10 , wherein the isolated host cell is a HeLa cell.