Colostral fractionation process
A method ( 100 ) and a system ( 200 ) for processing mammalian bodily fluids to isolate target peptides and proteins. The method can include the steps of passing a mammalian bodily fluid through at least one ion exchange column ( 204 ). The ion exchange column can include an anionic resin and/or a cationic resin. The ion exchange column can be selected to remove large particles at a first pH level selected to remove such large particles at a maximum fluid pressure of 30 pounds per square inch (psi), for example less than 10 psi. The large particles can be released from the ion exchange column with a rinse solution having a pH selected for releasing the large particles. The pH of the fluid then can be adjusted to be a value less than 5.0, and the fluid can be filtered with a microfilter or ultrafilter ( 210 ).
1. A method for separating the proline-rich polypeptides essentially free of peptides and proteins of about 10,000 Daltons or greater from the globular proteins having a molecular weight of between 10,000 and 500,000 Daltons in bovine colostrum, the method comprising the steps of:
(a) subjecting at least a portion of a bovine colostrum sample to at least one separation procedure comprising an ion exchange separation to yield separated proline-rich polypeptides essentially free of peptides and proteins of about 10,000 Daltons or greater and separated globular proteins having a molecular weight of between 10,000 and 500,000 Daltons, wherein no more than about 10% of the separated proline-rich polypeptides are denatured; and
(b) collecting the separated proline-rich polypeptides;
wherein at least 90% of the separated proline-rich polypeptides have a molecular weight of no more than about 2,500 Daltons.
2. The method of claim 1 , wherein the step (a) is performed at a pressure of 30 psi or less.
3. The method of claim 1 , wherein the step (a) is performed at a pressure of 10 psi or less.
4. The method of claim 1 , wherein the at least one separation procedure further comprises a size exclusion separation.
5. The method of claim 1 , wherein the step (a) comprises changing the pH of the at least a portion of the colostrum.