Early-inflorescence-preferred regulatory elements and uses thereof
The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for regulatory elements providing expression preferentially in the inflorescence meristem and developing floral tissues. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided, as well as expression constructs, vectors, and transformed cells and plants.
1. An isolated nucleic acid comprising a functional regulatory element represented by a sequence selected from the group consisting of:
(a) SEQ ID NO: 1;
(b) SEQ ID NO: 3;
(c) SEQ ID NO: 4;
(d) SEQ ID NO: 5; and
(e) a fragment of SEQ ID NO: 1 comprising nucleotides 990-2197.
2. An expression cassette comprising a first nucleotide sequence which is a regulatory element, and a second nucleotide sequence operably linked to the regulatory element, wherein the regulatory element is represented by a sequence selected from the group consisting of:
(a) SEQ ID NO: 1;
(b) SEQ ID NO: 3;
(c) SEQ ID NO: 4;
(d) SEQ ID NO: 5; and
(e) a fragment of SEQ ID NO: 1 comprising nucleotides 990-2197.
3. A transformation vector comprising the expression cassette of claim 2 .
4. A plant stably transformed with the expression cassette of claim 2 .
5. The plant of claim 4 , wherein said plant is a monocot.
6. The plant of claim 5 , wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
7. Seed of the plant of claim 4 , wherein said seed comprise the expression cassette of claim 2 .
8. A method for selectively expressing a nucleotide sequence in a plant cell, the method comprising transforming a plant cell with a transformation vector comprising an expression cassette, said expression cassette comprising a first nucleotide sequence which is a regulatory element and a second nucleotide sequence operably linked to the regulatory element, wherein the regulatory element is represented by a sequence selected from the group consisting of:
(a) SEQ ID NO: 1;
(b) SEQ ID NO: 3;
(c) SEQ ID NO: 4;
(d) SEQ ID NO: 5; and
(e) a fragment of SEQ ID NO: 1 comprising nucleotides 990-2197.
9. The method of claim 8 wherein the plant cell is comprised by callus tissue.
10. The method of claim 8 further comprising regenerating a stably transformed plant from said transformed plant cell.
11. A stably transformed plant created by the method of claim 10 .
12. The plant of claim 11 wherein the phenotype of said stably transformed plant is altered relative to a nontransgenic isoline.
13. The plant of claim 12 wherein the phenotype of one or more reproductive structures is altered relative to a nontransgenic isoline.
14. The method of claim 8 , wherein said second nucleotide sequence encodes a gene involved in fatty acid synthesis.
15. A plant cell stably transformed with an expression cassette comprising a first nucleotide sequence which is a regulatory element and a second nucleotide sequence operably linked to the regulatory element, wherein the regulatory element is represented by a sequence selected from the group consisting of:
(a) SEQ ID NO: 1;
(b) SEQ ID NO: 3;
(c) SEQ ID NO: 4;
(d) SEQ ID NO: 5; and
(e) a fragment of SEQ ID NO: 1 comprising nucleotides 990-2197.
16. The plant cell of claim 15 , wherein said plant cell is from a monocot.
17. The plant cell of claim 16 , wherein said plant cell is from maize, wheat, rice, barley, sorghum, or rye.