Use of microparticles with adsorbed antigen to stimulate immune responses
The use of poly(lactide) or poly(lactide-co-glycolide) microparticles with adsorbed antigen is disclosed. The microparticles are useful for enhancing CTL responses to a selected antigen.
1. A method of producing a microparticle with adsorbed antigen comprising:
(a) forming a microparticle that comprises a poly(α-hydroxy acid) by a method comprising (i) forming an emulsion comprising said poly(α-hydroxy acid), water and an organic solvent and (ii) evaporating said solvent; and
(b) adsorbing an antigen derived from a viral pathogen to the surface of said microparticle.
2. The method of claim 1 , wherein the microparticle comprises a poly(α-hydroxy acid) selected from the group consisting of poly(L-lactide), poly(D, L-lactide) and poly(D,L-lactide-co-glycolide).
3. The method of claim 2 , wherein the microparticle comprises poly(D,L-lactide-co-glycolide).
4. The method of claim 1 , wherein the antigen comprises HIV gp 120.
5. The method of claim 1 , wherein the antigen comprises HIV p24gag.
6. The method of claim 1 , wherein the antigen comprises Influenza A hemagglutinin antigen.
7. The method of claim 1 wherein said emulsion is a water-in-oil-in-water emulsion.
8. The method of claim 1 , wherein said antigen comprises a hepatitis B viral antigen.
9. The method of claim 1 , wherein said antigen comprises a hepatitis C viral antigen.
10. The method of claim 1 , wherein said antigen comprises an influenza A viral antigen.
11. The method of claim 1 , wherein said antigen comprises an HIV antigen.
12. The method of claim 1 , wherein the microparticle has a diameter between 500 nanometers and 10 microns.
13. The method of any of claims 1 and 7 , wherein the poly(α-hydroxy acid) is a poly(lactide-co-glycolide).
14. The method of claim 7 , wherein the microparticle comprises a poly(α-hydroxy acid) selected from the group consisting of poly(L-lactide), poly(D,L-lactide) and poly(D,L-lactide-co-glycolide).
15. The method of claim 7 , wherein the poly(α-hydroxy acid) is poly(D,L-lactide-co-glycolide).
16. The method of claim 7 , wherein the antigen comprises HIV gp 120.
17. The method of claim 7 , wherein the antigen comprises HIV p24gag.
18. The method of claim 7 , wherein the antigen comprises Influenza A hemagglutinin antigen.
19. The method of claim 7 , wherein said antigen comprises a hepatitis B viral antigen.
20. The method of claim 7 , wherein said antigen comprises a hepatitis C viral antigen.
21. The method of claim 7 , wherein said antigen comprises an influenza A viral antigen.
22. The method of claim 7 , wherein said antigen comprises an HIV antigen.
23. The method of claim 7 , wherein the microparticle has a diameter between 500 nanometers and 10 microns.
24. The method of any of claims 4 - 6 , 8 , 9 , 10 , 11 , and 12 , wherein the poly(α-hydroxy acid) is a poly(lactide-co-glycolide).
25. The method of claim 7 , wherein said emulsion comprises an emulsion stabilizer.
26. The method of claim 25 , wherein said emulsion stabilizer is polyvinyl alcohol.