Optimizing glycan processing plants
The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus 5 containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.
1. A method of producing a plant cell expressing a heterologous glycoprotein that is reduced in xylose and fucose, comprising introducing into the plant cell a first expression cassette and a second expression cassette, wherein the first expression cassette comprises a nucleic acid comprising SEQ ID No:3 encoding a hybrid enzyme, and the second expression cassette comprises nucleic acid encoding a heterologous glycoprotein, and expressing the hybrid enzyme and the heterologous glycoprotein, wherein the expressed glycoprotein is reduced in xylose and fucose when compared to a glycoprotein expressed in a plant cell in the absence of the hybrid enzyme.
2. A method of producing a plant cell expressing a heterologous glycoprotein that is reduced in xylose and fucose, comprising introducing into the plant cell a first expression cassette and a second expression cassette, wherein the first expression cassette comprises nucleic acid encoding a hybrid enzyme comprising an amino acid sequence comprising SEQ ID No:4, and the second expression cassette comprises nucleic acid encoding a heterologous glycoprotein, and expressing the hybrid enzyme and the heterologous glycoprotein, wherein the expressed glycoprotein is reduced in xylose and fucose when compared to a glycoprotein expressed in a plant cell in the absence of the hybrid enzyme.