Fluorescence detection apparatus
Fluorescence detection apparatus detects fluorescence from a fluorescent object. The apparatus includes a light source configured to irradiate the fluorescent object with light, a shutter configured to block the light, from the light source, directed to the fluorescent object, an optical output measuring unit arranged in an optical path between the shutter and the light source, an image pickup element configured to detect the fluorescence from the fluorescent object and to capture a noise image, and a changing unit configured to change at least one of an accumulation time of the image pickup element and an open-close time of the shutter. The changing unit calculates the accumulation time for capturing the noise image using the measurement result of the optical output measuring unit, and corrects a captured fluorescent image generated by detecting the fluorescence, using the noise image captured during the accumulation time calculated by the calculation unit.
1. An emission detection apparatus that detects emission from an emission object, comprising:
a light source configured to irradiate the emission object with light;
a shutter configured to block the light from the light source;
an optical output measuring unit configured to detect the light from the light source;
an image pickup element configured to detect the emission from the emission object; and
a changing unit configured to change at least one of an open-close time of the shutter and an accumulation time of the image pickup element, on the basis of the measurement result of the optical output measuring unit.
2. A fluorescence detection apparatus that detects fluorescence from a fluorescent object, comprising:
a light source configured to irradiate the fluorescent object with light;
a shutter configured to block the light, from the light source, directed to the fluorescent object;
an optical output measuring unit arranged in an optical path between the shutter and the light source;
an image pickup element configured to detect the fluorescence from the fluorescent object and to capture a noise image; and
a changing unit configured to change at least one of an accumulation time of the image pickup element and an open-close time of the shutter,
wherein the changing unit includes,
a calculation unit configured to calculate the accumulation time for capturing the noise image on the basis of the measurement result of the optical output measuring unit, and
a correction unit configured to correct a captured fluorescent image that is generated by detecting the fluorescence, using the noise image captured during the accumulation time calculated by the calculation unit.
3. The fluorescence detection apparatus according to claim 2 ,
wherein the light source emits exciting light that excites the fluorescent object, and
wherein the fluorescence detection apparatus further comprises an image pickup unit having a fluorescent filter configured to transmit the fluorescence from the fluorescent object, to guide the fluorescence to the image pickup unit, and to block the exciting light.
4. The fluorescence detection apparatus according to claim 2 , wherein the light source is a laser source.
5. The fluorescence detection apparatus according to claim 2 , wherein the changing unit allows a memory to store a waiting time, measures a time from when the light source is turned ON, and after the waiting time has elapsed, transmits a signal to start measuring the optical output from the light source.
6. The fluorescence detection apparatus according to claim 5 , wherein the noise image is captured before the fluorescent object is set and after the waiting time of the light source has elapsed.
7. The fluorescence detection apparatus according to claim 2 , wherein a plurality of the fluorescent objects are provided, and a plurality of captured images obtained from the plurality of fluorescent objects are corrected on the basis of the noise image, used as a common noise image.
8. The fluorescence detection apparatus according to claim 2 , wherein a plurality of images are captured prior to detection of fluorescence from the fluorescent object, and a single noise image is generated using the plurality of images.
9. The fluorescence detection apparatus according to claim 2 ,
wherein the optical output measuring unit is capable of accumulating light during the irradiation of the light on the fluorescent object, and transmits a signal to close the shutter to the changing unit in response to the signal from the optical output measuring unit.
10. The fluorescence detection apparatus according to claim 9 , wherein the changing unit transmits a signal to close the shutter to the shutter in response to the signal from the optical output measuring unit, and transmits a signal to terminate the accumulation of the image pickup element to the image pickup element, and
wherein the changing unit includes,
a unit configured to measure and store the accumulation time for capturing the fluorescence, and
a second correction unit configured to correct the noise image by the accumulation time for capturing the fluorescence.
11. The emission detection apparatus according to claim 1 , further comprising at least one of:
a DNA amplifying system configured to amplify an inserted DNA of living matter,
a labeling system configured to bind the amplified DNA with a marker fluorescent molecule, and
a hybridization system configured to bind the labeled DNA with a probe of a DNA chip.
12. An apparatus that acquires a dark image, comprising:
an optical output measuring unit configured to detect light from a light source; and
a changing unit configured to change an accumulation time of an image pickup element on the basis of the measurement result of the optical output measuring unit,
wherein the image pickup element acquires the dark image on the basis of the accumulation time.