IP Library Granted Patent US 7,632,462
Granted Patent B2
US 7,632,462 · App. 10/476,185 · Granted Dec 15, 2009

Assay system

Assignee: Axis-Shield ASA
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Quick Facts
Patent No.
US 7,632,462
App. No.
10/476,185
Granted
Dec 15, 2009
Kind
B2
Abstract

An assay apparatus comprising: i) an assay cartridge ( 52,53 ) comprising at least one well ( 57 - 62 ) and a pipette ( 50 ) positionable in at least one said well; ii) a holder arranged to receive said cartridge; iii) drive means operable to position said pipette in selected wells of said cartridge; iv) a gas pressure applicator couplable to said pipette whereby to cause liquid flow through said membrane; and v) a radiation detector operable to detect radiation from a well of said cartridge or from said pipette.

Claims (20)

1. An assay cartridge comprising at least two wells and a membrane-tipped pipette positionable in at least two of said wells, said membrane-tipped pipette having a proximal end and a distal end, said distal end being closed by a liquid permeable membrane, wherein said cartridge further comprises a capillary-tipped pipette.

2. An assay cartridge as claimed in claim 1 wherein said membrane-tipped pipette is closed by a sloping liquid permeable membrane.

3. An assay cartridge as claimed in claim 2 wherein said sloping membrane lies in a plane at an angle of 20 to 40° to the axis of said membrane-tipped pipette to which it is attached.

4. An assay cartridge as claimed in claim 1 wherein said a membrane-tipped pipette has a membrane-tipped end is of rectangular cross section.

5. An assay cartridge as claimed in claim 1 wherein said cartridge comprises detachable base and cap members, said wells being disposed in said base member and said cap member being arranged to carry said membrane-tipped pipette positionable in at least two of said wells.

6. An assay cartridge as claimed in claim 5 wherein said cap member comprises means to receives said capillary-tipped pipette.

7. An assay cartridge as claimed in claim 5 wherein at least one of said wells is sealed at its upper end by a frangible seal and wherein said cap member is provided with a cutter arranged to pierce said seal.

8. An assay cartridge as claimed in claim 5 wherein said base member comprises an absorbent wiper arranged to wipe the outside of said capillary-tipped pipette inserted therein.

9. An assay cartridge as claimed in claim 1 wherein said membrane-tipped pipette the proximal end whereof is closed by a piercable self-sealing membrane.

10. An assay cartridge as claimed in claim 1 wherein the wells in said cartridge are arranged in a linear array.

11. An assay cartridge as claimed in claim 1 wherein at least one of said wells contains an assay reagent.

12. An assay cartridge as claimed in claim 1 wherein said capillary tip is provided with a detachable sleeve.

13. An assay cartridge as claimed in claim 1 wherein at least one said well has two parallel planar side walls joined by a base wall comprising at least one planar face a normal to the surface whereof is coplanar to and non-perpendicular to normals to the parallel planar surfaces of said side walls.

14. An assay device comprising a) a cartridge holder capable of receiving an assay cartridge comprising at least two wells and a membrane-tipped pipette positionable in at least two of said wells, said membrane-tipped pipette having a proximal end and a distal end, said distal end being closed by a liquid permeable membrane; b) drive means operable to position said membrane-tipped pipette of said cartridge in selected wells of said cartridge; c) a gas pressure applicator couplable to said membrane-tipped pipette of said cartridge whereby to cause liquid flow therethrough; and d) a radiation detector operable to detect radiation from said well of said cartridge or from said membrane-tipped pipette thereof

15. A device as claimed in claim 14 wherein said radiation detector comprises a digital camera.

16. A device as claimed in claim 14 further comprising a light source arranged to illuminate said cartridge.

17. A device as claimed in claim 14 further comprising a magnet.

18. A device as claimed in claim 14 further comprising a heater arranged to heat said cartridge.

19. A device as claimed in claim 14 further comprising a controller arranged to control assay performance by said apparatus.

20. A device as claimed in claim 14 wherein said gas pressure applicator comprises a piston disposed within a cylindrical housing and a drive motor arranged to drive said piston.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 9, 2004
From: HOLTLUND, JOSTEIN; BORCH, STIG MORTEN; SEIM, THORSTEIN; JANSON, TORE; TON, HEGE; KARLSON, JAN ROGER; LAUVSTAD, INGER LISE
To: AXIS-SHIELD ASA
Reel/Frame 015276/0181 →
Priority Claims (2)
GB 0111360.4 · May 9, 2001 · national
GB 0130359.3 · Dec 19, 2001 · national
Continuity (1)
Related Publication 20040161368A1 · Aug 19, 2004