Methods and reagents for dendritic localization of polynucleotides
View Patent ↗A recombinant nucleic acid comprising nucleotides corresponding to a 3.2 kb 3′ untranslated region present in mouse calcium calmodulin kinase II alpha subunit-encoding mRNA is provided. An isolated nucleic acid which hybridizes to the 3′ untranslated region of mouse calcium calmodulin kinase II alpha subunit-encoding mRNA under conditions comprising two post-hybridization washes of 15 minutes each at 42° C. in 0.2× standard sodium citrate buffer is also provided.
1. A recombinant nucleic acid comprising a 3.2 kb 3′ untranslated region of mouse calcium calmodulin kinase II alpha subunit-encoding mRNA and mRNA encoding a protein.
2. An isolated nucleic acid which hybridizes to the 3′ untranslated region of mouse calcium calmodulin kinase II alpha subunit-encoding mRNA under conditions comprising two post-hybridization washes of 15 minutes each at 42° C. in 0.2× standard sodium citrate buffer.
3. A nucleic acid which is a plasmid designated pMM281 deposited with the American Type Culture Collection under Accession No. 98581.
4. A cell comprising the nucleic acid of any of claims 1 , 2 and 3 .
5. The cell of claim 4 , wherein the cell is a bacterial cell, an insect cell, or a mammalian cell.