High transgene expression of a pseudotyped adeno-associated virus type
The present invention related to methods and compositions comprising recombinant vectors comprising chimeric capsids and recombinant pseudotyped vectors with non-native capsid protein(s). The recombinant vectors of the invention confer an altered tropism that permits selective targeting of desired cells.
1. A recombinant virion comprising SEQ ID NO:11.
2. The recombinant virion of claim 1 , wherein the virion further comprises a transgene.
3. The recombinant virion of claim 1 , wherein the virion further comprises a transgene expression cassette.
4. The recombinant virion of claim 3 , wherein the transgene expression cassette comprises a transgene.
5. The recombinant virion of claim 3 , wherein the transgene expression cassette comprises a tissue-specific promoter.
6. The recombinant virion of claim 5 , wherein the tissue-specific promoter is operable in cells of the central nervous system.
7. The recombinant virion of claim 5 , wherein the tissue-specific promoter is a neuron-specific promoter.
8. The recombinant virion of claim 5 , wherein the tissue-specific promoter is a pyramidal cell-specific promoter.
9. The recombinant virion of claim 1 , wherein the virion further comprises control elements that are functional in a brain neuron.
10. The recombinant virion of claim 1 , wherein the virion further comprises control elements that are functional in a striatum.
11. The recombinant virion of claim 1 , wherein the virion further comprises control elements that are functional in a hippocampus.