Therapeutic and diagnostic cloned MHC-unrestricted receptor specific for the MUC1 tumor associated antigen
View Patent ↗The invention provides an isolated nucleic acid encoding a receptor, other than an immunoglobulin, wherein the receptor binds to a MUC1 tumor antigen independently of an major histocompatibility complex (MHC). The invention provides a method of activating a signaling pathway and/or killing a cancer cell using a receptor that is similar to or is a T cell receptor.
1. An isolated nucleic acid encoding a receptor, other than an immunoglobulin, wherein the receptor binds to a MUC 1 tumor antigen independently of a major histocompatibility complex (MHC), wherein the receptor
a. has a first amino acid sequence comprising the sequence of the complementarity determining regions (CDRs) of MA Vα23 shown in FIG. 1 (SEQ ID NO:1), and
b. has a second amino acid sequence comprising the sequence of the complementarity determining regions (CDRs) MA Vβ8.3 shown in FIG. 1 (SEQ ID NO:2).
2. An isolated nucleic acid encoding a receptor, other than an immunoglobulin, wherein the receptor binds to a MUC 1 tumor antigen independently of a major histocompatibility complex (MHC), wherein the receptor comprises a portion of the zeta-chain of CD3.
3. The isolated nucleic acid of claim 2 , wherein the portion of the zeta-chain comprises the transmembrane spanning region of CD3.