Screening method for genes of brewing yeast
Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae , (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae ; and (D) analyzing the selected gene.
1. An isolated DNA selected from the group consisting of
a DNA having SEQ ID NO: 1, and,
a DNA which hybridizes to a polynucleotide consisting of the nucleotide complementary to the nucleotide sequence of SEQ ID NO: 1 under stringent conditions.
2. The isolated DNA of claim 1 consisting of SEQ ID NO: 1.
3. An isolated DNA selected from the group consisting of
DNA which encodes a polypeptide having SEQ ID NO: 3, and
DNA which encodes a polypeptide having an amino acid sequence in which one to 20 amino acid residues are deleted, substituted, added or a combination thereof, in an amino acid sequence having SEQ ID NO: 3.
4. The isolated DNA of claim 3 , consisting of a DNA encoding a polypeptide consisting of SEQ ID NO: 3.
5. An isolated recombinant vector comprising the DNA of any of claims 1 - 4 .
6. The recombinant vector of claim 5 , wherein a promoter, a terminator, or both are placed adjacent to said DNA.
7. The recombinant vector of claim 6 , wherein said promoter is a constitutive promoter.
8. The recombinant vector of claim 6 , wherein said promoter is a glyceraldehyde-3 -phosphate dehydrogenase promoter.
9. An isolated transformant comprising the DNA of any of claims 1 - 4 .
10. The transformant of claim 9 , wherein said transformant is a yeast of genus Saccharomyces.