Methods for measuring the insulin receptor α subunit
View Patent ↗Presence of free insulin receptor α-subunit in blood was discovered. Furthermore, methods for measuring the insulin receptor α-subunit was provided, the method comprising the steps of contacting the insulin receptor α-subunit in a blood sample with an antibody recognizing the insulin receptor α-subunit, and detecting the binding between the two. Measurement of the free insulin receptor α-subunit in the blood is useful for evaluating risk factors for diabetes. In addition, the measurement methods of the present invention showed that concentrations of the free insulin receptor α-subunit in the blood of diabetes or cancer patients are significantly high. Free insulin receptor α-subunit in blood is useful as a marker for diabetes or cancer.
1. A method for diagnosing diabetes, wherein the method comprises the steps of:
a) measuring the amount of a free insulin receptor α-subunit in a biological sample of a subject;
b) comparing the amount of the free insulin receptor α-subunit with that of a control; and
c) determining the subject to have diabetes when the amount of free insulin receptor α-subunit in the biological sample of the subject is greater than that of the control.
2. The method for diagnosis of claim 1 , wherein the biological sample is a blood sample.
3. The method for diagnosis of claim 2 , wherein the amount of the free insulin receptor α-subunit is measured by the steps of: (1) contacting a blood sample with an antibody recognizing the insulin receptor α-subunit; (2) detecting binding of said antibody to the insulin receptor α-subunit present in blood; and (3) determining the amount of free insulin receptor α-subunit in blood based on the level of binding detected between said antibody and subunit.