Plasmid encoding human BMP-7
The present invention relates to recombinant vectors expressing the BMP-7 polypeptide in host cells and to pharmaceutical compositions comprising such recombinant vectors. The invention also encompasses methods for prevention and/or treatment of both acute and chronic renal failure in mammals, advantageously in humans, dogs and cats, by intra-vascular kidney administration of the recombinant vectors and pharmaceutical compositions of the invention.
1. A recombinant plasmid vector comprising a nucleic acid sequence encoding a human BMP-7 polypeptide operatively linked to a promoter, wherein the BMP-7 polypeptide has at least 98% similarity or identity with SEQ ID NO: 15.
2. The recombinant plasmid vector according to claim 1 , wherein the nucleic acid sequence encoding the human BMP-7 polypeptide is selected from the group consisting of SEQ ID NO: 13 and SEQ ID NO: 14.
3. The recombinant plasmid vector according to claim 1 , wherein the plasmid is pMEB038 and has the nucleotide sequence of SEQ ID NO: 16, or wherein the plasmid comprises the nucleic acid sequence encoding the human BMP-7 polypeptide inserted into the VR1012 plasmid.
4. The recombinant plasmid vector of claim 1 , wherein the human BMP-7 polypeptide has at least 99% similarity or identity with SEQ ID NO: 15.
5. The recombinant plasmid vector of claim 1 , wherein the human BMP-7 polypeptide has the amino acid sequence of SEQ ID NO: 15.
6. The recombinant plasmid vector of claim 1 , wherein the human BMP-7 polypeptide has the amino acid sequence of SEQ ID NO: 15 and is encoded by nucleotides 1 to 1296 of SEQ ID NO: 13.
7. The recombinant plasmid vector of claim 1 , wherein the human BMP-7 polypeptide has the amino acid sequence of residues 30-431 of SEQ ID NO: 15 and is encoded by nucleotides 88 to 1296 of SEQ ID NO: 13.
8. A pharmaceutical composition comprising a recombinant plasmid vector according to any one of claims 1 or 2 - 7 , and optionally at least one pharmaceutically or veterinarily acceptable carrier, excipient, or vehicle.
9. The recombinant plasmid vector according to claim 1 , wherein the human BMP-7 polypeptide comprises a heterologous signal peptide.