Apparatuses and methods for isolating nucleic acid
The present invention is an apparatus and method for purifying and isolating nucleic acid. The system uses one or more automated stations to perform steps of the method, including loading a sample onto a solid support in a processing container, purifying the sample, and isolating nucleic acid from the sample. A pump or vacuum may be used in the apparatus of the present invention for supplying fluids to the sample. A pump, vacuum or centrifuge may be used to separate impurities from the sample or to separate nucleic acid from the solid support. Exemplary solid supports and sample processing containers are disclosed.
1. A method for isolating a nucleic acid comprising:
introducing a fluid sample containing the nucleic acid into a sample processing container through an outlet of the sample processing container;
introducing a purification/wash solution into the sample processing container through the outlet of the sample processing container;
forcing the purification/wash solution out of the sample processing container;
introducing an elution solution into the sample processing container; and
forcing the elution solution out of the sample processing container, thereby isolating the nucleic acid.
2. The method of claim 1 , further comprising heating the sample processing container after introducing the fluid sample.
3. The method of claim 2 , wherein the heating is by microwaves or ultrasound waves.
4. The method of claim 1 , further comprising introducing a lysing solution into the sample processing container.
5. The method of claim 1 , wherein the elution solution is introduced into the outlet of the sample processing container.
6. The method of claim 1 , wherein the elution solution is introduced into the inlet of the sample processing container.
7. The method of claim 1 , wherein the fluid sample is selected from the group consisting of body fluids, body wastes, body excretions, and blood.
8. The method of claim 1 , wherein the forcing is performed by vacuum pressure, positive pressure, gravity, centrifugation, or mechanical agitation.
9. The method of claim 1 , wherein the fluid sample is mixed with the purification/wash solution and elution solution by the induction of fluid motion through the solid support by alternately dispensing and aspirating the purification/wash solution and elution solution through the septum.