Method for production of (1S)-3-chloro-1-(2-thienyl)-propan-1-ol using alcohol dehydrogenase from thermoanaerobacter
A process for preparing optically active (1S)-3-chloro-1-(2-thienyl)propan-1-ol of the formula I by, in a medium comprising 3-chloro-1-(2-thienyl)propan-1-one of the formula II reducing this compound to the compound of the formula I by means of an alcohol dehydrogenase from the Thermoanaerobacter genus, and isolating the product formed in substantially enantiomerically pure form.
1. A process for preparing optically active (1S)-3-chloro-1-(2-thienyl)propan-1-ol having formula (I)
comprising
(1) reducing 3-chloro-1-(2-thienyl)propan-1-one having formula (II)
to said optically active (1 S)-3-chloro-1-(2-thienyl)propan-1-ol having formula (I) with an alcohol dehydrogenase from the Thermoanaerobacter genus in the presence of a cofactor; and
(2) isolating said 3-chloro-1-(2-thienyl)propan-1-one having formula (II) formed in substantially enantiomerically pure form;
wherein said reduction is conducted in a medium comprising said 3-chloro-1-(2-thienyl) propan-1-one having formula (II).
2. The process of claim 1 , wherein said cofactor is NADPH.
3. The process of claim 2 , wherein said cofactor is regenerated in the reduction.
4. The process of claim 3 , wherein said regeneration is carried out by the enzymatic oxidation of isopropanol.