Insulin production methods and pro-insulin constructs
Novel pro-insulin having specific amino acid and/or nucleic acid modifications suitable for improved methods of insulin production are provided. Novel and highly efficient processes for preparing the pro-insulin preparations and preparations containing them are also disclosed. The novel pro-insulin preparations may be converted into human insulin useful in therapeutic preparations. Novel peptides of the C-peptide, and N terminus, including RREAEALQVGQVELGGGPGAGSLQPLALEGSLQAR (SEQ ID NO: 32), and MHHHHHHGGR (SEQ ID NO: 2) respectively are provided, as well as the unique nucleic acid molecules encoding them.
1. A composition comprising a peptide comprising:
B chain—RREAEALQVGQVELGGGPGAGSLOPLALEGSLQAR (SEQ ID NO: 32)—
A chain,
wherein said A chain and said B chain are native human insulin chains.
2. The composition of claim 1 wherein the peptide further comprising a His-tag at the N-terminus of the B-chain.
3. The composition of claim 2 wherein the His-tag comprises SEQ ID NO: 2.
4. A pharmaceutical product comprising a composition as defined in claim 1 and a pharmaceutically acceptable carrier solution.