Sequences diagnostic for foot and mouth disease

1. A method for detecting the presence of FMDV in a sample, the method comprising:

(a) performing RT-PCR amplification of the sample using at least one primer pair selected from the group consisting of:

(i) SEQ ID NOs:16 and 17,

(ii) SEQ ID NOs:16 and 18,

(iii) SEQ ID NOs:16 and 19, and

(iv) SEQ ID NOs:16 and 20,

to produce an RT-PCR amplification result; and

(b) examining the RT-PCR amplification result of step (a) to detect for an amplification product of the primer pair, whereby a positive detection of the amplification product indicates the presence of FMDV in the sample.

2. The method of claim 1 , wherein in step (b) a melting curve analysis is used to detect for an amplification product.

3. The method of claim 1 , further comprising a step of extracting RNA from the sample prior to said step (a).

4. A primer for use in PCR amplification for detection of FMDV consisting essentially of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, or SEQ ID NO:20.

5. A kit for detection of FMDV, comprising:

(a) at least one primer pair selected from the group consisting of:

(i) SEQ ID NOs:16 and 17,

(ii) SEQ ID NOs:16 and 18,

(iii) SEQ ID NOs:16 and 19, and

(iv) SEQ ID NOs:16 and 20;

(b) reverse transcriptase; and

(c) thermostable DNA polymerase.

6. A replication composition for use in performance of RT-PCR, comprising:

(a) at least one primer pair selected from the group consisting of:

(i) SEQ ID NOs:16 and 17,

(ii) SEQ ID NOs:16 and 18,

(iii) SEQ ID NOs:16 and 19, and

(iv) SEQ ID NOs:16 and 20;

(b) reverse transcriptase; and

(c) thermostable DNA polymerase.

7. A tablet comprising the replication composition of claim 6 .

8. A kit for detection of FMDV in a sample, comprising the tablet of claim 6 .