System for optically analyzing a substance with a selected single-wavelength
View Patent ↗A microfluidic analysis system for optically analyzing a substance includes a light source having a plurality of selectable single-wavelength light sources, a substance presentation member optically coupled to the light source, and an optical detection system associated with the substance presentation member.
1. A method of using a light source carousel including a plurality of selectable single-wavelength light sources, comprising:
selecting a desired single-wavelength light source from said plurality of selectable single-wavelength light sources;
illuminating an analyte with said desired single-wavelength light source; and
detecting a wavelength associated with said analyte when illuminated by said selected single-wavelength;
said selecting of the desired single-wavelength light source being accomplished by rotating said carousel which supports said plurality of single-wavelength light sources so as to optically couple one of said light source with said analyte, and rotating a micro-fluidic disk bearing said analyte to position said analyte for illumination;
wherein said carousel bearing said light sources and said micro-fluidic disk are concentric and rotate on a common axis.
2. The method of claim 1 , wherein said selecting a desired single-wavelength light source from said plurality of selectable single-wavelength light sources is at least partially based on an absorption or emission characteristic of said analyte.
3. The method of claim 1 , wherein said selecting a desired single-wavelength light source from said plurality of selectable single-wavelength light sources comprises:
identifying a peak or plateau in an absorption or emission spectrum of said analyte; and
selecting a single-wavelength light source with a radiation wavelength associated with said absorption or emission spectrum peak or plateau.
4. The method of claim 1 , further comprising:
illuminating a plurality of analytes simultaneously with said plurality of selectable single-wavelength light sources; and
simultaneously detecting wavelengths associated with said plurality of illuminated analytes.
5. The method of claim 4 , wherein said simultaneously detecting wavelengths associated with said plurality of illuminated analytes comprises detecting said wavelengths with a charge coupled device.
6. The method of claim 1 , wherein there is a one-to-one correspondence between said plurality of light sources and cuvettes on said micro-fluidic disk.
7. The method of claim 1 , further comprising additionally rotating said carousel to optically couple said analyte to a series of said light sources, in which said light sources emit different wavelengths of light.
8. The method of claim 1 , wherein selecting a desired single-wavelength light source comprises translating a substrate bearing said plurality of light sources with respect to said analyte so as to optically couple a selected light source with said analyte.
9. The method of claim 1 , further comprising using a light pipe to optically couple said plurality of light sources with said analyte.
10. A method of using a light source carousel including a plurality of selectable single-wavelength light sources to analyze number of analytes, said method comprising:
rotating said carousel which supports said plurality of single-wavelength light sources so as to optically couple one of said light sources with said analytes;
rotating a micro-fluidic disk bearing said analytes in a number of cuvettes defined in the micro-fluidic disk to position said analytes for illumination;
illuminating said analytes with said optically coupled light source; and
detecting light from said illuminated analytes,
in which there is a one-to-one correspondence between said plurality of light sources and cuvettes on said micro-fluidic disk.
11. The method of claim 10 , wherein said carousel bearing said light sources and said micro-fluidic disk are concentric and rotate on a common axis.
12. The method of claim 10 , further comprising additionally rotating said carousel to optically couple said analyte to a series of said light sources, in which said light sources emit different wavelengths of light.
13. The method of claim 10 , wherein detecting light from said illuminated analyte further comprises detecting analyte chemical reaction products in said sample of said analyte.