Isolated plasma and method for hyperimmunisation and plasma collection
The present invention relates to isolated canine animal plasma, a method for isolating canine animal plasma, plasma obtained form an immunised or hyperimmunised canine animal and treating a canine animal with the isolated canine animal plasma. The method includes the step of selecting a canine animal having a blood group compatible with a recipient canine animal having an unmatched blood group, namely, selecting a canine animal for a blood group that does not cause plasma transfusion reaction and/or haemolysis. In one form, the method includes the step of immunising or hyperimmunising a canine animal plasma donor with one or more antigens of a canine animal pathogen. The pathogen is preferably a bacteria or virus.
1. A method of isolating plasma from a canine animal including the steps of:
(I) selecting a donor canine animal having a blood group compatible with a recipient canine animal having an unmatched blood group, wherein the donor canine animal is selected for a phenotype lacking anti-globulin antibodies;
(II) collecting blood from the donor canine animal after administering a heat-killed E. coli antigen to said donor canine animal; and
(III) isolating plasma from blood collected in step (II).
2. The method of claim 1 wherein the donor canine animal is selected for a phenotype lacking at least one Dog Erythrocyte Antigen.
3. The method of claim 2 wherein the donor canine animal is negative for Dog Erythrocyte Antigen 1.1.
4. The method of claim 3 wherein the donor canine animal is negative for Dog Erythrocyte Antigen 1.2.
5. The method of claim 4 wherein the donor canine animal is negative for Dog Erythrocyte Antigen 7.
6. The method of claim 1 wherein step (II) further includes the steps of:
(a) inserting a blood collecting catheter into a vein of the donor canine animal;
(b) attaching the blood collecting catheter to a cell separator capable of separating blood into an isolated plasma component and an isolated blood cell component;
(c) collecting blood from the donor canine animal via the blood collection catheter;
(d) separating the blood into the isolated plasma component and the isolated blood cell component;
(e) collecting the isolated plasma component;
(f) stopping the collecting of blood;
(g) returning the blood cell component to the donor canine animal; and
(h) repeating steps (c)-(g).
7. The method of claim 1 , wherein said heat-killed E. coli antigen is an E. coli J5 antigen.
8. A method of producing hyperimmunised canine animal plasma including the steps of:
(1) selecting a donor canine animal having a blood group compatible with a recipient canine animal having an unmatched blood group, wherein said donor canine animal is characterised by a phenotype negative for anti-globulin antibodies;
(2) administering to the donor canine animal a heat-killed E. coli antigen thereby inducing an immune response in said donor canine animal;
(3) administering to said donor canine animal a heat-killed E. coli antigen during said immune response; and
(4) isolating plasma from said donor canine animal.
9. The method of claim 8 wherein said donor canine animal is further characterised by a phenotype negative for at least one Dog Erythrocyte Antigen.
10. The method of claim 9 wherein said donor canine animal is further characterised by a phenotype negative for Dog Erythrocyte Antigen 1.1.
11. The method of claim 9 wherein said donor canine animal is further characterised by a phenotype negative for Dog Erythrocyte Antigen 1.2.
12. The method of claim 9 wherein said donor canine animal is further characterised by a phenotype negative for Dog Erythrocyte Antigen 7.
13. The method of any one of claims 8 - 12 , wherein the heat-killed E. coli antigen is an E. coli J5 antigen.