Multimeric oxidoreductases
Described are multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate. The complexes having a dehydrogenase subunit and a cytochrome C subunit. Also described are polynucleotides coding for the multimeric complexes and methods of use thereof.
1. An isolated polynucleotide coding for a multimeric oxidoreductase complex, said complex consisting essentially of an alpha subunit, a gamma subunit and a cytochrome C subunit, wherein
the alpha subunit has dehydrogenase activity and has the amino acid sequence of
SEQ ID NO: 19 or a sequence having at least 95% sequence identity thereto;
the gamma subunit has the amino acid sequence of SEQ ID NO: 57 or a sequence having at least 95% sequence identity thereto; and
the cytochrome C subunit has the amino acid sequence of SEQ ID NO: 38 or a sequence having at least 95% sequence identity thereto.
2. The isolated polynucleotide of claim 1 , wherein the multimeric oxidoreductase complex is coded for by an operon.
3. A vector comprising the isolated polynucleotide of claim 1 .
4. The vector of claim 3 comprising SEQ ID NO: 115, SEQ ID NO: 77 and SEQ ID NO: 96.
5. A bacterial host cell transformed with the vector of claim 3 .
6. A vector comprising the isolated polynucleotide of claim 2 .