Drought and high light tolerant transgenic plants
View Patent ↗Transformed plants and/or plant cells having increased tolerance to high light, drought, water-deficit, and/or other stress-inducing conditions are provided. Plants and plant cells expressing or over-expressing an exogenous nucleic acid encoding a polypeptide involved in a CET pathway, including but not limited to, a PGR5 polypeptide, and methods of making the transformed plants and plant cells are also provided.
1. A transformed plant cell comprising: an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide, wherein the PGR5 polypeptide increases tolerance of the transformed plant cell, or a plant grown therefrom, relative to a corresponding wild-type plant cell or plant, to one or more conditions selected from: high light, water-deficit, and drought.
2. The transformed plant cell of claim 1 , wherein the PGR5 polypeptide is selected from:
an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
3. The transformed plant cell of claim 2 , wherein the exogenous nucleic acid encodes for a PGR5 polypeptide selected from: the Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3 and the Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5.
4. The transformed plant cell of claim 1 , further comprising a nucleic acid encoding a promoter, wherein the promoter controls expression of the exogenous nucleic acid.
5. The transformed plant cell of claim 4 , wherein the promoter directs over-expression of the exogenous nucleic acid relative to expression of a PGR5 nucleic acid in a corresponding wild-type cell.
6. The transformed plant cell of claim 4 , wherein the promoter is selected from: a stress-inducible promoter, a tissue-specific promoter, and a constitutive promoter.
7. The transformed plant cell of claim 4 , wherein the promoter is a cauliflower mosaic virus 35S promoter.
8. The transformed plant cell of claim 1 , further comprising a nucleic acid encoding a selectable marker.
9. The transformed plant cell of claim 1 , wherein the plant cell is a dicot plant cell.
10. The transformed plant cell of claim 1 , wherein the plant cell is an Arabidopsis thaliana cell.
11. The transformed plant cell of claim 1 , wherein the plant cell is naturally light-sensitive.
12. The transformed plant cell of claim 1 , wherein the plant cell is naturally drought-sensitive.
13. A transgenic plant grown from the plant cell of claim 1 .
14. A transformed plant cell comprising: an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide, wherein the PGR5 polypeptide increases the activity of PGR5-dependent cyclic electron transport in the transformed plant cell or a plant grown therefrom relative to a corresponding wild-type plant cell or plant.
15. The transformed plant cell of claim 14 , wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and
functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
16. A transformed plant cell comprising a recombinant nucleic acid comprising:
an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide, and
a nucleic acid encoding a promoter, wherein the promoter induces over-expression of the recombinant nucleic-acid relative to the expression of a PGR5 nucleic acid in a corresponding wild-type plant cell, and wherein the over-expression of the recombinant nucleic acid increases tolerance of the transformed plant cell, or a plant grown therefrom, to one or more conditions selected from: high light, water-deficit, and drought.
17. The transformed plant cell of claim 16 ,
wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
18. A transgenic plant comprising: an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide, wherein the PGR5 polypeptide increases tolerance of the transformed plant, relative to a corresponding wild-type plant, to conditions selected from: high light, water-deficit, and drought.
19. The transgenic plant of claim 18 , wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having an the amino acid sequence of SEQ ID NO: 5; and
functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
20. The transgenic plant of claim 19 , wherein the exogenous nucleic acid encodes for a polypeptide selected from: the Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3 and the Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5.
21. The transgenic plant of claim 18 , further comprising a nucleic acid encoding a promoter, wherein the promoter controls expression of the PGR5 polypeptide.
22. The transgenic plant of claim 21 , wherein the promoter induces higher levels of expression of PGR5 in the transformed plant than in an equivalent wild-type plant that does not comprise the exogenous nucleic acid encoding for PGR5 or the promoter.
23. The transgenic plant of claim 21 , wherein the promoter is selected from: a stress-inducible promoter, a tissue-specific promoter, and a constitutive promoter.
24. The transgenic plant of claim 21 , wherein the promoter is a cauliflower mosaic virus 35S promoter.
25. The transgenic plant of claim 18 , further comprising a nucleic acid encoding a selectable marker.
26. The transgenic plant of claim 18 , wherein the plant is a dicot.
27. The transgenic plant of claim 18 , wherein the plant is Arabidopsis thaliana.
28. The transgenic plant of claim 18 , wherein the plant is naturally light-sensitive.
29. The transgenic plant of claim 18 , wherein the plant is naturally drought-sensitive.
30. A transgenic plant comprising: an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide, wherein the expression of PGR5 increases the activity of PGR5 dependent cyclic electron transport in the transgenic plant relative to a corresponding wild-type plant.
31. The transgenic plant of claim 30 , wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
32. A transformed plant comprising a recombinant nucleic acid comprising:
an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide and
a nucleic acid encoding a promoter, wherein the promoter induces over-expression of PGR5 relative to a corresponding wild-type plant and wherein the over-expression of PGR5 increases tolerance of the transformed plant cell, or a plant grown therefrom, to one or more conditions selected from: high light, water-deficit, and drought.
33. The A transformed plant of claim 32 ,
wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
34. A method of producing a transformed plant cell comprising:
introducing into a plant cell an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide, and
expressing the PGR5 polypeptide in the cell, wherein the transformed plant cell has increased tolerance, relative to a corresponding wild-type plant cell, to one or more conditions selected from: high light, water-deficit, and drought.
35. The method of producing a transformed plant cell of claim 34 ,
wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
36. A method of producing a transgenic plant comprising:
introducing into a plant cell an exogenous nucleic acid that encodes a proton gradient regulation 5 (PGR5) polypeptide;
expressing the PGR5 polypeptide in the cell; and
cultivating the cell to generate a plant, wherein the transformed plant has increased tolerance, relative to a corresponding wild-type plant, to one or more conditions selected from: high light, water-deficit, and drought.
37. The A method of producing a transgenic plant of claim 36 ,
wherein the PGR5 polypeptide is selected from: an Arabidopsis thaliana PGR5 (AtPRG5) polypeptide having the amino acid sequence of SEQ ID NO: 3; functional variants of AtPRG5 and homologs of AtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 3; a Pinus taeda PGR5 (PtPGR5) polypeptide having the amino acid sequence of SEQ ID NO: 5; and functional variants of PtPRG5 and homologs of PtPRG5 having an amino acid sequence with at least 59% sequence identity to SEQ ID NO: 5.
38. The transformed plant cell of claim 3 , wherein the AtPGR5 polypeptide has the amino acid sequence of SEQ ID NO: 3.
39. The transformed plant cell of claim 3 , wherein the PtPGR5 polypeptide has an the amino acid sequence of SEQ ID NO: 5.