Interactive variable pathlength device
This disclosure relates generally to a sampling device, and, more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms, and methods for using such a device.
1. A method of determining the concentration of a sample comprising:
(a) placing the sample solution in a vessel;
(b) placing a probe within sample;
(c) taking an absorbance reading;
(d) moving the probe relative to the vessel with a unidirectional sliding mechanism by a predetermined increment such that a preselected path length through the solution is obtained such that a preselected path length through the solution is obtained and taking an absorbance reading at a predetermined wavelength;
(e) repeating steps (c) and (d) sequentially one or more times;
(f) generating a regression line from the absorbance values and path length such that a slope of the regression line is obtained; and,
(g) determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the sample.
2. A method of determining the concentration of a sample comprising:
(a) placing the sample solution in a vessel;
(b) moving a probe relative to the vessel such that the probe makes contact with the bottom of the vessel;
(c) moving the probe relative to the vessel with a unidirectional sliding mechanism such that the probe moves from the bottom of the vessel through the sample by a predetermined increment such that a preselected path length through the solution is obtained;
(d) taking an absorbance reading at a predetermined wavelength;
(e) repeating steps (c) and (d) sequentially one or more times;
(f) generating a regression line from the absorbance and path length such that a slope of the regression line is obtained; and,
(g) determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the sample.
3. The method of claim 1 wherein the predetermine increment is the same for each iteration.
4. The method of claim 1 wherein the predetermined increment is from about 0.005 mm to about 50 mm.
5. The method of claim 1 wherein the predetermined increment is from about 0.0002 mm to about 10 mm.
6. The method of claim 2 wherein the predetermine increment is the same for each iteration.
7. The method of claim 2 wherein the predetermined increment is from about 0.005 mm to about 50 mm.
8. The method of claim 2 wherein the predetermined increment is from about 0.0002 mm to about 10 mm.
9. The method of claim 1 wherein the regression line has an R 2 value of from around 0.99950 to about 0.99999.
10. The method of claim 2 wherein the regression line has an R 2 value of from around 0.99950 to about 0.9999.
11. The method of claim 1 wherein the regression line has an R 2 value of from around 0.99990 to about 0.99999.
12. The method of claim 2 wherein the regression line has an R 2 value of from around 0.99990 to about 0.99999.
13. An instrument for determine the concentration of a sample at multiple path lengths comprising:
(a) a light source operably linked to a probe;
(b) a sample vessel that can contain the sample;
(c) a motor operably linked to the sample vessel such that the sample vessel can be moved relative to the probe to provide variable path lengths;
(d) a probe that can carry electromagnetic radiation that can be moved relative to the sample vessel by the motor;
(e) a unidirectional sliding mechanism operably linked to the probe;
(f) a detector located below the sample vessel that can detect electromagnetic radiation disposed such that the detector is substantially perpendicular to the electromagnetic radiation emanating from the probe and such that the electromagnetic radiation moves through the sample and is detected by the detector; and,
(g) software for calculating the concentration of the sample based on the information provided by the detector at the predetermined path lengths;
comprising generating a regression line from the absorbance and path length such that a slope of the regression line is obtained; and determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the sample.
14. The instrument of claim 13 , wherein the unidirectional sliding mechanism is passively locked.
15. The instrument of claim 13 , wherein the unidirectional sliding mechanism is actively locked by a user.
16. The instrument of claim 13 , wherein the unidirectional sliding mechanism is operably linked to the probe.
17. The instrument of claim 13 , wherein the unidirectional sliding mechanism is operably linked to the sample vessel.
18. The instrument of claim 13 , wherein the path lengths are between about 0.2 μm to about 1.0 μm.
19. The instrument of claim 13 , wherein the path lengths are between about 0.2 μm to about 1.0 μm.
20. The instrument of claim 13 , wherein the motor is operably linked to the sample vessel though a sample vessel stage.
21. The instrument of claim 13 , wherein the motor is computer controlled.
22. The instrument of claim 13 , wherein the software can calculate the concentration based on deriving the slope of a line based on the ratio of path length to absorbance values.
23. The instrument of claim 22 , wherein the slope of the line is derived while the values are being measured.