Methods and materials for making and using transgenic dicamba-degrading organisms
View Patent ↗The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) of the invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation.
1. A recombinant DNA molecule, consisting essentially of a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one substitution.
2. A recombinant DNA molecule, consisting essentially of a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one addition.
3. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one substitution.
4. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one addition.
5. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one substitution and by one addition.
6. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence comprising SEQ ID NO:3.
7. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence comprises SEQ ID NO:2.
8. A transgenic plant cell that has been transformed with the plant transformation vector of claim 3 .
9. A transgenic plant cell that has been transformed with the plant transformation vector of claim 4 .
10. A transgenic plant cell that has been transformed with the plant transformation vector of claim 5 .
11. A transgenic plant cell that has been transformed with the plant transformation vector of claim 6 .
12. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 3 .
13. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 4 .
14. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 5 .
15. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 6 .
16. A transgenic seed from a transgenic plant according to claim 12 .
17. A transgenic seed from a transgenic plant according to claim 13 .
18. A transgenic seed from a transgenic plant according to claim 14 .
19. A transgenic seed from a transgenic plant according to claim 15 .