IP Library › Granted Patent US 7,820,437
Granted Patent B2
US 7,820,437 · App. 12/040,408 · Granted Oct 26, 2010

Constitutively open hERG (Kv11.1) mutant channels

Assignee: Wisconsin Alumni Research Foundation
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Quick Facts
Patent No.
US 7,820,437
App. No.
12/040,408
Granted
Oct 26, 2010
Kind
B2
Abstract

We disclose a cell having double mutations of the hERG gene that lead to charge reversal amino acid substitutions at residues 466 and 534 of the wild type Kv11.1 channel protein. These double charge reversal mutations result in cells having constitutively open Kv11.1 channels. Such cells could be used in a method of testing development-stage drugs and other compounds for Kv11.1 channel block activity.

Claims (7)

1. A cell comprising at least one constitutively open Kv11.1 channel wherein the Kv11.1 channel comprises at least two mutations, wherein amino acid residues of opposite charge substitute for residues 466 and 534 of the wild type Kv11.1 amino acid sequence disclosed in SEQ ID NO:1.

2. The cell of claim 1 wherein the pair of charge reversal mutations is selected from the group consisting of D466K/R534D and D466K/R534E.

3. The cell of claim 1 wherein the pair of charge reversal mutations is selected from the group consisting of D466R/R534D, D466R/R534E, D466H/R534D, and D466H/R534E.

4. The cell of claim 1 wherein the cell is a mammalian cell.

5. The cell of claim 4 wherein the cell is a HEK293 cell.

6. The cell of claim 4 wherein the cell is selected from the group consisting of a COS7 cell, a CHO cell, a native heart cell, and a cardiomyocyte.

7. A cell population comprising more than one of the cells of claim 1 .

Assignments (5)
CONFIRMATORY LICENSE Recorded Jul 23, 2010
From: UNIVERSITY OF WISCONSIN MADISON
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 024729/0478 →
CORRECTIVE ASSIGNMENT TO CORRECT THE APPLICATIONS ASSIGNED. APPLICATION NUMBER 12/040,404 SHOULD BE REMOVED FROM THE COVER SHEET PREVIOUSLY RECORDED ON REEL 022205 FRAME 0141. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT OF APPLICATION NUMBER 12/040,408 FROM UNIVERSITY OF WISCONSIN MADISON TO NATIONAL INSTITUTES OF HEALTH (NIH).. Recorded May 21, 2010
From: UNIVERSITY OF WISCONSIN MADISON
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS)
Reel/Frame 024422/0722 →
CORRECTIVE ASSIGNMENT TO CORRECT THE APPLICATIONS ASSIGNED PREVIOUSLY RECORDED ON REEL 022205 FRAME 0142. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT OF APPLICATION NUMBER 12/040,408 FROM UNIVERSITY OF WISCONSIN MADISON TO NATIONAL INSTITUTES OF HEALTH (NIH). Recorded May 19, 2010
From: UNIVERSITY OF WISCONSIN MADISON
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS)
Reel/Frame 024409/0603 →
CONFIRMATORY LICENSE Recorded Feb 4, 2009
From: UNIVERSITY OF WISCONSIN MADISON
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 022205/0141 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 10, 2008
From: JANUARY, CRAIG T; KLEMENS, CHRISTINE A; ANSON, BLAKE D; DELISLE, BRIAN P
To: WISCONSIN ALUMNI RESEARCH FOUNDATION
Reel/Frame 021955/0120 →
Continuity (2)
Provisional Application 6089269100 · Mar 2, 2007
Related Publication 20090035804A1 · Feb 5, 2009