Compositions and methods are provided for detection and treatment of Porphyromonas gingivalis infection.
1. A method for detecting the presence of an invasive Porphyromonas gingivalis infection in an animal comprising contacting a test sample from the animal with a purified polypeptide comprising the polypeptide sequence set forth as SEQ ID NO:17; and detecting immunocomplexes comprising the purified polypeptide comprising the polypeptide sequence set forth as SEQ ID NO:17 and an antibody or fragment thereof in the test sample, wherein detection of the immunocomplexes indicates the presence of invasive Porphyromonas gingivalis infection.
2. The method of claim 1 , wherein the method further comprises detecting the amount of immunocomplexes.
3. The method of claim 1 , wherein the test sample is serum, blood, saliva, or plaque.
4. The method of claim 1 , wherein the polypeptide is immobilized to a solid support.
5. The method of claim 1 , wherein the polypeptide is labeled.
6. The method of claim 2 , wherein the detection is by radioimmunoassay, enzyme-linked immunosorbent assay, immunohistochemical assay or immunoenzyme-assay.
7. A method for detecting an invasive Porphyromonas gingivalis polypeptide in a test sample comprising contacting the test sample with an antibody or fragment thereof that specifically binds a polypeptide consisting of the polypeptide sequence set forth as SEQ ID NO:17, and detecting immunocomplexes comprising the invasive P. gingivalis polypeptide and the antibody or fragment thereof, wherein detection of the immunocomplexes indicates the presence of the invasive Porphyromonas gingivalis polypeptide.
8. The method of claim 7 , wherein the method further comprises detecting the amount of immunocomplexes.
9. The method of claim 7 , wherein the test sample is a serum, blood, saliva, or plaque.
10. The method of claim 7 , wherein the antibody or fragment thereof is immobilized to a solid support.
11. The method of claim 7 , wherein the antibody or fragment thereof is labeled.
12. The method of claim 7 , wherein the detection is by radioimmunoassay, enzyme-linked immunosorbent assay, immunohistochemical, or immunoenzyme-assay.