Detection of sterilisation vapour condensation point
A method of detecting bacterial kill using a vaporized sterilant includes the steps of generating a sterilant/water vapour and supplying the vapour to a sealed space to be sterilized. The supply of sterilant/water vapour is continued to raise the concentration of the sterilant in the sealed space, and the concentration of the sterilant in the vapour is monitored to determine when the rate of change of the concentration falls to a predetermined minimal level indicating that condensation has occurred resulting in kill of any bacteria present in the space. Supply of sterilant/water vapour to the space is then terminated.
1. A method of detecting bacterial kill using a vaporized sterilant comprising the steps of:
generating a sterilant/water vapour and supplying the vapour in a sealed space to be sterilized,
continuing to supply the sterilant/water vapour to raise a concentration of the sterilant in the sealed spaced,
monitoring concentration of the sterilant in the vapour by measuring the concentration of sterilant in the vapour,
determining from said measured concentration values when a rate of change of the concentration of the sterilant falls to a predetermined minimal level indicating that condensation of the vapour has occurred resulting in kill of any bacteria present in the space, and
terminating supply of the sterilant/water vapour.
2. A method as claimed in claim 1 , wherein said predetermined minimal level of the rate of change of the concentration of the sterilant is a positive rate of change.
3. A method as claimed in claim 2 , wherein a temperature of an atmosphere in the enclosed space is monitored and the supply of sterilant/water vapour to the sealed space is continued whilst the temperature rises until the rate of change of the concentration of the sterilant falls to said predetermined minimal level.
4. A method as claimed in claim 2 , wherein the sterilant/water vapour is supplied to the chamber to cause a multiple number of atmosphere changes in the chamber in raising the concentration of the sterilant in the chamber.
5. A method as claimed in claim 1 , wherein the predetermined minimal level of the rate of change of the concentration of the sterilant is a negative rate of change.
6. A method as claimed in claim 5 , wherein a temperature of an atmosphere in the enclosed space is monitored and the supply of sterilant/water vapour to the sealed space is continued whilst the temperature rises until the rate of change of the concentration of the sterilant falls to said predetermined minimal level.
7. A method as claimed in claim 5 , wherein the sterilant/water vapour is supplied to the chamber to cause a multiple number of atmosphere changes in the chamber in raising the concentration of the sterilant in the chamber.
8. A method as claimed in claim 1 , wherein a temperature of an atmosphere in the enclosed space is monitored and the supply of sterilant/water vapour to the sealed space is continued whilst the temperature rises until the rate of change of the concentration of the sterilant falls to said predetermined minimal level.
9. A method as claimed in claim 8 , wherein the sterilant/water vapour is supplied to the chamber to cause a multiple number of atmosphere changes in the chamber in raising the concentration of the sterilant in the chamber.
10. A method as claimed in claim 1 , wherein the sterilant/water vapour is supplied to the chamber to cause a multiple number of atmosphere changes in the chamber in raising the concentration of the sterilant in the chamber.
11. A method of detecting bacterial kill using a vaporized sterilant comprising the steps of:
generating a sterilant/water vapour and supplying the vapour in a sealed space to be sterilized;
continuing to supply the sterilant/water vapour to raise a concentration of the sterilant in the sealed spaced;
monitoring concentration of the sterilant in the vapour by directly measuring the concentration of sterilant in the vapour;
determining from the measured concentration values when a rate of change of the concentration of the sterilant falls to a predetermined minimal level indicating that condensation of the vapour has occurred in the sealed space; and
terminating supply of the sterilant/water vapour when the rate of change of the concentration of the sterilant falls to the predetermined minimal level.