Antibodies against type A botulinum neurotoxin
View Patent ↗Antibodies for binding epitopes of BoNT/A and hybridomas which produce such antibodies are described. The antibodies of the present invention can be used in a method for detecting BoNT/A in a sample and/or in a method for purifying BoNT/A from an impure solution. In addition, the antibodies can be used for passive immunization against BoNT/A intoxication or as intoxication therapy. Another aspect of the invention is a kit for detecting BoNT/A in a sample.
1. Monoclonal antibody 6C2-4, produced by the hybridoma cell line deposited with the American Type Culture Collection under accession number ATCC PTA-970.
2. A composition comprising the monoclonal antibody of claim 1 .
3. A pharmaceutical composition comprising the monoclonal antibody of claim 1 .
4. The continuous hybridoma cell line deposited with the American Type Culture Collection under accession number ATCC PTA-970.
5. A method for detecting BoNT/A said method comprising:
(i) incubating a sample with an effective amount of one or more monoclonal antibody against BoNT/A comprising 6C2-4 produced by the hybridoma cell line deposited with the American Type Culture Collection under accession number ATCC PTA 970, under conditions which allow the formation of an antibody-BoNT/A complex; and
(ii) detecting the antibody-BoNT/A complex wherein the presence or absence of the complex indicates the presence or absence of BoNT/A in the sample.
6. A method for detecting BoNT/A according to claim 5 wherein, said sample is water, a biological sample, an environmental sample, or a food product.
7. A kit for detecting BoNT/A in a sample, said kit comprising:
(1) a container containing monoclonal antibody 6C2-4produced by the hybridoma cell line deposited with the American Type Culture Collection under the accession number ATCC PTA-970; and, optionally
(2) instructions for using the antibody for the purpose of binding to BoNT/A to form an immunological complex and detecting the formation of the immunological complex such that presence or absence of immunological complex correlates with presence or absence of BoNT/A in said sample.
8. A method for capturing BoNT/A from a sample, said method comprising contacting said sample with the monoclonal antibody 6C2-4, produced by the hybridoma cell line deposited with the American Type Culture Collection under the accession number ATCC PTA-970, and isolating the complex formed between the BoNT/A in the sample and the monoclonal antibody.
9. The method according to claim 8 wherein said sample is selected from the group consisting of: a biological sample, an environmental sample and a food product.