Control of fruit dehiscence in plants by
View Patent ↗The present application provides methods and compositions that modulate fruit dehiscence in plants.
1. A non-naturally occurring plant of a Brassicaceae species produced by:
(a) providing plants expressing an IND1 polypeptide that is at least 60% identical to SEQ ID NO:2, comprises a basic helix-loop-helix (HLH) domain, and complements an Arabidopsis ind1 mutant phenotype when introduced into an Arabidopsis ind1 mutant plant;
(b) treating the plants with a chemical mutagen or ionizing radiation; and
(c) selecting a plant with delayed fruit dehiscence and comprising a mutant IND1 allele encoding a mutant IND1 polypeptide that does not complement an Arabidopsis ind1 mutant phenotype when introduced into an Arabidopsis ind1 mutant plant, wherein the plant has delayed fruit dehiscence compared to a plant in which the IND1 allele is not mutated.
2. The plant of claim 1 , wherein the mutant IND1 polypeptide is truncated before the basic HLH domain.
3. The plant of claim 1 , wherein the mutant IND1 polypeptide comprises a point mutation or a deletion in the basic HLH domain.
4. The plant of claim 1 , wherein the IND1 polypeptide in (a) has the amino acid sequence of SEQ ID NO:2.
5. A progeny of the plant of claim 1 , in which the expression of the IND1 polypeptide is suppressed.