Nontypeable
The invention relates to a mutation within the sap operon of an avirulent clone of a nontypeable strain of Haemophilus influenzae (NTHi). The invention also relates to the NTHi sap operon genes and the polypeptides encoded by these polynucleotide sequences. The invention also relates to a novel 110 kDa NTHi outer membrane protein and the polynucleotide that encodes this outer membrane protein. Methods of screening for NTHi infection, and treating and preventing NTHi related disorders are also contemplated.
1. An isolated polypeptide comprising an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4.
2. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO: 10.
3. A composition comprising a polypeptide of claim 1 and a pharmaceutically acceptable carrier.
4. A method for eliciting an immune response to NTHi bacteria comprising administering an immunogenic dose of a polypeptide of claim 1 to a patient at risk of NTHi bacterial infection.
5. A composition comprising a polypeptide of claim 2 and a pharmaceutically acceptable carrier.