Process for production of
View Patent ↗A nucleic acid sequence encoding all or part of an 18-19 kDa Helicobater pylori protein is described to which immunoreactivity is detected in H. pylori negative individuals. A process for the production of a recombinant form of this protein and its use, particularly as a vaccine to provide immunological protection against H. pylori infection are also described.
1. A process for the production of a recombinant nucleic acid comprising culturing a host cell comprising a vector, said vector comprising the nucleic acid sequence of SEQ ID NO:2 encoding a protein comprising an amino acid sequence of SEQ ID NO:1, and isolating the recombinant nucleic acid therefrom.
2. A process for the production of a recombinant H. pylori protein comprising culturing a host cell comprising a vector, said vector comprising a nucleic acid sequence encoding a protein comprising SEQ ID NO:1, wherein said nucleic acid sequence comprises SEQ ID NO:2, and isolating the protein produced therefrom.
3. A process for producing a recombinant Helicobacter pylori protein, said process comprising of expressing a Helicobacter pylori protein comprising the amino acid sequence of SEQ ID NO:1 from a vector comprising a recombinant nucleic acid sequence comprising SEQ ID NO:2.
4. A process for producing a recombinant Helicobacter pylori protein, said process comprising:
amplifying a nucleic acid encoding a Helicobacter pylori protein comprising a molecular weight of 18-19 kDa from Helicobacter pylori DNA using a primer comprising SEQ ID NO: 3 and a primer comprising SEQ ID NO: 4;
inserting said nucleic acid into a vector; and
expressing said Helicobacter pylori protein from said vector.
5. The process of claim 4 wherein said nucleic acid comprises SEQ ID NO: 2.