Bacterium consortium, bio-electrochemical device and a process for quick and rapid estimation of biological oxygen demand
View Patent ↗The present invention deals with a device for quick estimation of biochemical oxygen demand of beverage waste water. This device consists of an immobilized microbial membrane attached to an electrode, multimeter and a laptop workstation installed with a developed software. BOD measurement of beverage waste water using this device is rapid, reproducible and effective as compared to conventional titration based methods. This device also excludes COD estimation as required for BOD estimation of waste water. This bio-electrochemical device may find wide commercial application in beverage industries emanating waste waters.
1. A process for quick estimation of BOD of beverage waste water using bio-electrochemical device, wherein the said process comprising the steps of:
(a) providing bio-electrochemical device comprising:
i) microbes immobilized membrane attached to a commercially available dissolved oxygen probe;
ii) multimeter; and
iii) a laptop workstation installed with software configured to take “change in current” values in real time and manipulate the values in desired graphical displays;
(b) applying an external polarization of −0.62 V to −0.67 V to the microbes immobilized membrane of said bio-electrochemical device;
(c) stabilizing the said membrane in 0.025-0.075 M phosphate buffer at pH 6.4-7.2 for 30-45 minutes followed by addition of glucose glutamic acid (GGA) in phosphate buffer;
(d) observing the stability of the immobilized microbial membrane through software using stabilized membrane of said device by measuring the change in oxygen concentration in terms of current covering a used range of GGA concentrations;
(e) calibrating the change in current for different concentrations of GGA as obtained from step (d) with the conventional BOD values;
(f) replacing the GGA solution with fresh phosphate buffer and stabilizing the assembly;
(g) adding the beverage waste water in 0.025-0.075 M phosphate buffer at pH 6.4-7.2 to maintain the percentage as determined by software;
(h) observing the change in oxygen concentration in terms of current with the help of software;
(i) calculating the BOD values using software with the help of calibration curve by putting the change in current as observed in step (h).
2. A process as claimed in claim 1 , wherein the waste water is collected from any beverage industry.
3. A process as claimed in claim 1 , wherein the said device is capable to estimate the load of BOD from waste water varying from 300-2250 mg/lit of BOD at various periods of time.