B7-H5, a costimulatory polypeptide
View Patent ↗B7-HS costimulatory polypeptides, nucleic acids encoding such polypeptides, and methods for using the polypeptides and nucleic acids to enhance a T cell response are provided herein.
1. An isolated polypeptide consisting of an amino acid sequence encoded by a nucleic acid sequence that encodes a protein consisting of an extracellular region of a protein, wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 936 of SEQ ID NO:2.
2. An isolated polypeptide consisting of all or part of amino acid 30 to amino acid 311 of SEQ ID NO:1 and including at least an V-like Ig domain consisting of amino acid 47 to amino acid 150 of SEQ ID NO:1.
3. The polypeptide of claim 1 , wherein the amino acid sequence comprises amino acid 40 to amino acid 190 of SEQ ID NO:1.
4. The isolated polypeptide of claim 1 , wherein the amino acid sequence comprises amino acid 35 to amino acid 190 of SEQ ID NO:1.
5. The isolated polypeptide of claim 1 , wherein the polypeptide has the ability to co-stimulate a T cell.
6. A fusion protein comprising a first domain and at least one additional domain,
wherein the first domain consists of an amino acid sequence encoded by a nucleic acid sequence that encodes a protein consisting of an extracellular region of a protein, wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 936 of SEQ ID NO:2 and
the at least one additional domain comprises an amino acid sequence that is unrelated to SEQ ID NO:1.
7. The fusion protein of claim 6 , wherein the at least one additional domain is selected from the group consisting of: a heterologous targeting or leader sequence and an amino acid sequence that facilitates purification, detection or solubility of the fusion protein.
8. The fusion protein of claim 6 , wherein the at least one additional domain is all or part of an immunoglobulin heavy chain constant region.
9. An isolated antigenic polypeptide consisting of at least 8 consecutive residues of a polypeptide consisting of amino acid 30 to amino acid 311 of SEQ ID NO:1.
10. The polypeptide of claim 1 , wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 570 of SEQ ID NO:2.
11. The polypeptide of claim 1 , wherein the amino acid sequence comprises amino acid 30 to amino acid residue 190 of SEQ ID NO:1.
12. The polypeptide of claim 2 , wherein the amino acid sequence comprises amino acid 40 to amino acid 311 of SEQ ID NO:1.
13. The polypeptide of claim 2 , wherein the amino acid sequence comprises amino acid 35 to amino acid 311 of SEQ ID NO:1.
14. The polypeptide of claim 2 , wherein the amino acid sequence comprises amino acid 30 to amino acid 311 of SEQ ID NO:1.
15. A fusion protein comprising a first domain and at least one additional domain, wherein the first domain consists of a polypeptide consisting of all or part of amino acid 30 to amino acid 311 of SEQ ID NO:1 and including at least an V-like Ig domain consisting of amino acid 47 to amino acid 150 of SEQ ID NO:1 and
the at least one additional domain comprises an amino acid sequence that is unrelated to SEQ ID NO:1.
16. The fusion protein of claim 15 , wherein the at least one additional domain is selected from the group consisting of: a heterologous targeting or leader sequence and an amino acid sequence that facilitates purification, detection or solubility of the fusion protein.
17. The fusion protein of claim 15 , wherein the at least one additional domain is all or part of an immunoglobulin heavy chain constant region.
18. The fusion protein of claim 6 , wherein the polypeptide comprises amino acid 40 to amino acid 190 of SEQ ID NO: 1.
19. The fusion protein of claim 6 , wherein the polypeptide comprises amino acid 35 to amino acid 190 of SEQ ID NO:1.
20. The fusion protein of claim 6 , wherein the polypeptide comprises amino acid 30 to amino acid 190 of SEQ ID NO:1.
21. The fusion protein of claim 15 , wherein the polypeptide comprises amino acid 40 to amino acid 311 of SEQ ID NO: 1.
22. The fusion protein of claim 15 , wherein the polypeptide comprises amino acid 35 to amino acid 311 of SEQ ID NO:1.
23. The fusion protein of claim 15 , wherein the polypeptide comprises amino acid 30 to amino acid 311 of SEQ ID NO:1.
24. An isolated polypeptide consisting of all or part of amino acid 30 to amino acid 309 of SEQ ID NO:3 and including at least a V-like Ig domain consisting of amino acid 47 to amino acid 149 of SEQ ID NO:3.
25. The fusion proteim of claim 6 , wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2 ×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 570 of SEQ ID NO:2.