PCSK9 polypeptide fragment
The present invention provide PCSK9 polypeptides, fragments thereof and methods of modulating PCSK9 phosphorylation and low density lipoprotein degradation.
1. A purified proprotein convertase subtilisin/kexin type 9 (PCSK9) polypeptide fragment consisting of the amino acid sequence VLALRSEEDGLAEAP (SEQ ID NO:2) or VLALRS(phos)EEDGLAEAP (SEQ ID NO:3), wherein (phos) indicates phosphorylation of the preceding amino acid.
2. The PCSK9 polypeptide fragment of claim 1 , consisting of the amino acid sequence VLALRSEEDGLAEAP (SEQ ID NO:2).
3. The PCSK9 polypeptide fragment of claim 1 , consisting of the amino acid sequence VLALRS(phos)EEDGLAEAP (SEQ ID NO:3).
4. A purified nucleotide sequence encoding the PCSK9 polypeptide fragment of claim 1 .
5. A composition comprising the PCSK9 polypeptide fragment according to claim 1 and a pharmaceutically acceptable carrier, diluent or excipient.
6. A kit comprising,
a) the PCSK9 polypeptide fragment of claim 1 ;
b) a nucleic acid encoding the PCSK9 polypeptide fragment of claim 1 ;
c) a combination of (a) and (b); or
d) a composition comprising (a), (b), or (c) and one or more pharmaceutically acceptable carriers, diluents, or excipients;
and optionally further comprising one or both of:
e) one or more devices for delivering or administering said PCSK9 polypeptide fragment or said composition comprising said PCSK9 polypeptide fragment; and
f) instructions for administering said PCSK9 polypeptide fragment or said composition comprising said PCSK9 polypeptide fragment.
7. A vector comprising the nucleotide sequence of claim 4 .