Polypeptide having Δ5 desaturating activity, polynucleotide coding for the polypeptide, and use thereof
The present invention relates to a polypeptide having a Δ5 fatty acid desaturation activity and a polynucleotide encoding the polypeptide as well as use thereof. For example, the present invention relates to a polypeptide, comprising (a) the amino acid sequence represented by SEQ ID NO: 2; or (b) the amino acid sequence in which one or more amino acids are substituted, deleted, inserted or added in the amino acid sequence represented by SEQ ID NO: 2, and having the Δ5 fatty acid desaturation activity; an antibody capable of binding to this polypeptide; a polynucleotide encoding the polypeptide; a vector comprising this polynucleotide; a cell or transformant introduced with the polynucleotide; a method of producing a fatty acid using the cell, and so on.
1. An isolated polynucleotide encoding a polypeptide having a Δ5 fatty acid desaturation activity, wherein the polypeptide comprises:
(a) the amino acid sequence of SEQ ID NO: 2; or
(b) the amino acid sequence that has been modified by substitution, deletion, insertion, and/or addition of one to ten amino acids of SEQ ID NO: 2.
2. An isolated polynucleotide, which is either (a) or (b) defined below:
(a) an isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1; or,
(b) an isolated polynucleotide hybridizable to (i) or (ii) below under stringent conditions:
(i) an isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1; or,
(ii) an isolated polynucleotide consisting of the nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1.
3. The polynucleotide of claim 1 , which is either (a) or (b) defined below:
(a) an isolated polynucleotide consisting of the 68th-1498th nucleotide sequence of SEQ ID NO: 1; or,
(b) an isolated polynucleotide hybridizable to either (i) or (ii) below under stringent conditions:
(i) an isolated polynucleotide consisting of the 68th-1498th nucleotide sequence of SEQ ID NO: 1; or,
(ii) an isolated polynucleotide consisting of the nucleotide sequence complementary to the 68th-1498th nucleotide sequence of SEQ ID NO: 1.
4. A vector comprising the polynucleotide of claim 1 .
5. A method of producing a polypeptide comprising administering to a cell the vector of claim 4 .
6. A non-human transformant transformed with the polynucleotide of claim 1 .
7. The transformant of 6 , in which the fatty acid composition is modified.
8. The transformant according to claim 6 , which is a plant or a progeny of a plant wherein the plant or the progeny is transformed with the polynucleotide of claim 3 , or a tissue derived therefrom.
9. The transformant of claim 8 , wherein the plant is tobacco or rice.
10. A method of producing a polypeptide comprising culturing the transformant of claim 6 .
11. A method of producing a fatty acid comprising extracting a fatty acid from the transformant of claim 6 .
12. A cell comprising the vector of claim 4 .
13. The cell of 12 , which is a cell of rice, tobacco or yeast.
14. A method of producing a polypeptide comprising culturing the cell of claim 12 .
15. A method of producing a fatty acid comprising introducing the polynucleotide of claim 1 into a yeast or a plant, and extracting a fatty acid from the yeast or the plant.
16. The method of producing a fatty acid of claim 11 , wherein the fatty acid is pinolenic acid or coniferonic acid.
17. A detector comprising the polynucleotide of claim 1 immobilized on a substrate.